3M Molecular Detection Assay 2 Manuel D'instructions page 4

• Do not exceed the recommended temperature setting on heater.
• Do not exceed the recommended heating time.
• Use an appropriate, calibrated thermometer to verify the 3M™ Molecular Detection Heat Block Insert temperature
(e.g., a partial immersion thermometer or digital thermocouple thermometer, not a total immersion thermometer.) The
thermometer must be placed in the designated location in the 3M Molecular Detection Heat Block Insert.
To reduce the risks associated with cross-contamination, including false-positive results:
• Use of sterile, aerosol barrier (filtered), molecular biology grade pipette tips is recommended.
• Use a new pipette tip for each sample transfer.
• Use Good Laboratory Practices to transfer the sample from the enrichment to the lysis tube. To avoid pipettor
contamination, the user may choose to add an intermediate transfer step. For example, the user can transfer each
enriched sample into a sterile tube.
• Use a molecular biology workstation containing germicidal lamp where available.
• Periodically decontaminate laboratory benches and equipment (pipettes, cap/decap tools, etc.) with a 1- 5% (v:v in
water) household bleach solution or DNA removal solution.
To reduce the risks associated with a false-positive result:
• Never open reagent tubes post amplification.
• Always dispose of the contaminated tubes by soaking in a 1-5% (v:v in water) household bleach solution for 1 hour and
away from the assay preparation area.
Consult the Safety Data Sheet for additional information and local regulations for disposal.
If you have questions about specific applications or procedures, please visit our website at www.3M.com/foodsafety or
contact your local 3M representative or distributor.
Limitation of Warranties / Limited Remedy
EXCEPT AS EXPRESSLY STATED IN A LIMITED WARRANTY SECTION OF INDIVIDUAL PRODUCT PACKAGING, 3M
DISCLAIMS ALL EXPRESS AND IMPLIED WARRANTIES, INCLUDING BUT NOT LIMITED TO, ANY WARRANTIES
OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE. If any 3M Food Safety Product is defective, 3M or its
authorized distributor will, at its option, replace or refund the purchase price of the product. These are your exclusive
remedies. You must promptly notify 3M within sixty days of discovery of any suspected defects in a product and return
it to 3M. Please call Customer Service (1-800-328-1671 in the U.S.) or your official 3M Food Safety representative for a
Returned Goods Authorization.
Limitation of 3M Liability
3M WILL NOT BE LIABLE FOR ANY LOSS OR DAMAGES, WHETHER DIRECT, INDIRECT, SPECIAL, INCIDENTAL OR
CONSEQUENTIAL DAMAGES, INCLUDING BUT NOT LIMITED TO LOST PROFITS. In no event shall 3M's liability under
any legal theory exceed the purchase price of the product alleged to be defective.
User Responsibility
Users are responsible for familiarizing themselves with product instructions and information. Visit our website at
www.3M.com/foodsafety, or contact your local 3M representative or distributor for more information.
When selecting a test method, it is important to recognize that external factors such as sampling methods, testing
protocols, sample preparation, handling, and laboratory technique may influence results.
It is the user's responsibility in selecting any test method or product to evaluate a sufficient number of samples with the
appropriate matrices and microbial challenges to satisfy the user that the chosen test method meets the user's criteria.
It is also the user's responsibility to determine that any test methods and results meet its customers' and suppliers'
requirements.
As with any test method, results obtained from use of any 3M Food Safety product do not constitute a guarantee of the
quality of the matrices or processes tested.
To help customers evaluate the method for various food matrices, 3M has developed the 3M™ Molecular Detection
Matrix Control kit. When needed, use the Matrix Control (MC) to determine if the matrix has the ability to impact the 3M
Molecular Detection Assay 2 - Listeria results. Test several samples representative of the matrix, i.e. samples obtained from
different origin, during any validation period when adopting the 3M method or when testing new or unknown matrices or
matrices that have undergone raw material or process changes.
A matrix can be defined as a type of product with intrinsic properties such as composition and process. Differences
between matrices may be as simple as the effects caused by differences in their processing or presentation for example,
raw vs. pasteurized; fresh vs. dried, etc.
CAUTION
NOTICE
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