3M Molecular Detection Assay 2 Manuel D'instructions page 12

Appendix A. Protocol Interruption: Storage and re-testing of heat-treated lysates
1. To store a heat-treated lysate, re-cap the lysis tube with a clean cap (see "LYSIS", 4.5)
2. Store at 4 to 8°C for up to 72 hours.
3. Prepare a stored sample for amplification by inverting 2-3 times to mix.
4. Decap the tubes.
5. Place the mixed lysate tubes on 3M Molecular Detection Heat Block Insert and heat at 100 ±1°C for 5 ±1 minutes.
6. Remove the rack of LS tubes from the heating block and allow to cool in the 3M Molecular Detection Chill Block Insert
at least 5 minutes and a maximum of 10 minutes.
7. Continue the protocol at the 'Amplification' section detailed above.
References:
1. US Food and Drug Administration Bacteriological Analysis Manual. Chapter 10: Detection and Enumeration of Listeria
monocytogenes in Foods. January 2016 Version.
2. US Department of Agriculture (USDA) FSIS Microbiology Laboratory Guidebook 8.08. Isolation and Identification of
Listeria monocytogenes from Red Meat, Poultry and Egg Products, and Environmental Samples. Effective Date: May 1,
2013.
3. ISO 11290-1. Microbiology of Food and Animal Feeding Stuffs – Horizontal Method for the Detection and Enumeration
of Listeria monocytogenes. Amendment 1, 2004-10-15.
4. ISO/IEC 17025. General requirements for the competence of testing and calibration laboratories.
5. ISO 7218. Microbiology of food and animal feeding stuffs – General rules for microbiological examination.
6. 3M. Installation Qualification (IQ) / Operational Qualification (OQ) Protocols and Instructions for 3M Molecular
Detection System.
7. ISO 18593. Microbiology of food and animal feeding stuffs – Horizontal methods for sampling techniques from surfaces
using contact plates and swabs.
8. ISO 16140-2 Microbiology of the food chain – Method validation – Part 2: Protocol for the validation of alternative
(proprietary) methods against a reference method.
9. ISO 6887. Microbiology of food and animal feeding stuffs – Preparation of test samples, initial suspension and decimal
dilutions for microbiological examination.
Explanation of Symbols
www.3M.com/foodsafety/symbols
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EN (English)