3M Molecular Detection Assay 2 Manuel D'instructions page 110

4. Åpne rørene.
5. Plasser de blandede lysatrørene på 3M Varmeblokkinnsats for molekylær deteksjon og varm ved 100 ± 1 °C i 5 ± 1
minutter.
6. Fjern det utildekkede stativet med LS-rør fra varmeblokken og la det avkjøles i 3M Kjøleblokkinnsats for molekylær
deteksjon i minst 5 minutter og maksimum 10 minutter.
7. Fortsett protokollen ved «Amplifikasjon»-delen beskrevet ovenfor.
Referanser:
1. US Food and Drug Administration Bacteriological Analysis Manual. Kapittel 10: Detection and Enumeration of Listeria
monocytogenes in Foods. Januari 2016-versjonen.
2. US Department of Agriculture (USDA) FSIS Microbiology Laboratory Guidebook 8.08. Isolation and Identification of
Listeria monocytogenes from Red Meat, Poultry and Egg Products, and Environmental Samples. Effektiv dato: 1. mai
2013.
3. ISO 11290-1. Microbiology of Food and Animal Feeding Stuffs – Horizontal Method for the Detection and Enumeration
of Listeria monocytogenes. Endring 1, 2004-10-15.
4. ISO/IEC 17025. General requirements for the competence of testing and calibration laboratories.
5. ISO 7218. Microbiology of food and animal feeding stuffs – General rules for microbiological examination.
6. 3M. Installation Qualification (IQ) / Operational Qualification (OQ) Protocols and Instructions for 3M Molecular
Detection System.
7. ISO 18593. Microbiology of food and animal feeding stuffs – Horizontal methods for sampling techniques from surfaces
using contact plates and swabs.
8. ISO 16140-2 Microbiology of the food chain – Method validation – Part 2: Protocol for the validation of alternative
(proprietary) methods against a reference method.
9. ISO 6887. Microbiology of food and animal feeding stuffs – Preparation of test samples, initial suspension and decimal
dilutions for microbiological examination.
Forklaring av symboler
www.3M.com/foodsafety/symbols
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