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Piccolo Xpress Manuel De L'opérateur page 32

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3.5.3
Lactate Dehydrogenase (LD) Assay
Blood cells contain significant levels of LD, and therefore all LD assays are sensitive to
hemolysis caused by release of LD from red blood cells. In the following circumstances,
the results are annotated to help interpret LD activity in the presence of small amounts of
hemolysis.
If HEM is greater than 50 and less than or equal to 100 mg/dL, the printed LD value is
followed by an
If HEM is greater than 100 and less than or equal to 150 mg/dL, the LD value is pre-
ceded by
If HEM is greater than 150, no result will be indicated, and only
3.5.4
Endogenous Substances
The analyzer judges the quality of each sample, and measures spectrophotometrically the concentra-
tions of endogenous substances for each run. The levels of Hemolysis (
Icterus (
) are indicated at the bottom of each result tape or on the touchscreen.
ICT
Levels are scored as 0, 1+, 2+, or 3+ depending on levels of each interferent. The following table
shows the relationship between these scores and the approximate concentration of each endogenous
substance in the sample.
Correlation of Sample Index vs Endogenous Substance Concentration
3-14
, indicating additional influence from hemolysis.
H
and followed by
<
H
Index
HEM
0
1+
2+
3+
LIP
0
1+
2+
3+
ICT
0
1+
2+
3+
Testing and Results
, indicating that the true LD recovery is less than reported.
Endogenous Substance
Concentration (mg/dL)
< 20
≥ 20 to < 45
≥ 45 to ≤ 181
> 181
< 150
≥ 150 and < 221
≥ 221 and ≤ 301
> 301
< 2.8
≥ 2.8 and < 5.5
≥ 5.5 and ≤ 10.5
> 10.5
will be printed.
HEM
), Lipemia (
HEM
LIP
), and

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