Incident Light Brightfield - Leica DM R Serie Mode D'emploi

As well as diffusing screen pair A, 1 2 diffusing
screen pair B, order no. 565 502, can be
supplied. Diffusing screen pair B contains 2
identical diffusing screens and is recommended
when the same illumination conditions are
required on both channels.

Incident light brightfield

Set the microscope illumination to medium
intensity (42.14 and 42.8).
Turn in a low power objective (e.g. 10x). Make
sure the front lenses of the objectives are clean!
Push in the diaphragm module (65.9) as far as
the stop (= channel I).
Close the field diaphragm (65.8). Open the
aperture diaphragm (65.12).
Using the stage clamp (48.9) and the coarse
focus control (42.12) or (44.2 and 44.3), position
the sample surface roughly in the focal plane (=
45 mm below the objective thread, see page 57).
Focus the object. The image of the closed field
diaphragm (65.8) makes it easier to find the
object surface.
See page 67 for tube and eyepiece setting.
Setting the field diaphragm:
Close the field diaphragm (65.8) until its edge
can just be seen within the observed object field
(49b). Put the two centering keys (1.5) into the
holes (65.7) and adjust until the edge of the field
diaphragm is concentric with the edge of the
field of view (49c). Centration of the closed field
diaphragm can also be performed with a
graticule e.g. with crosslines.
Open the field diaphragm (49d) until it just
disappears from the field of view.
This setting of the field diaphragm is retained for
all objectives.
If the diaphragm module HC RF is pulled out
as far as the st clickstop (= channel II), the field
and aperture diaphragms are fixed, see chart on
p. 96.
The field diaphragm only has to be readjusted
when eyepieces with different field numbers are
used, when the secondary magnification is
altered with a magnification changer or zoom
system, or for photography and filming.
Narrowing the field diaphragm usually improves
the contrast.
For interchangeable stages only: The centering
keys can be kept in the stage bracket (42.11 or
13.3) after use.
The aperture diaphragm
resolution, contrast and depth of field of the
microscope image.
It must be set carefully and must not be used to
adjust image intensity.
(65.12) affects
97