Phase Contrast - Leica DM R Serie Mode D'emploi

Phase contrast

Like transmitted light darkfield and transmitted
light interference contrast ICT, phase contrast is
used to produce high-contrast images of
unstained specimens.
Turn the phase contrast objective (engraving
PH) with the lowest magnification (generally
10x) into the light path and focus the specimen.
If you have trouble finding the specimen plane:
temporarily stop down the aperture diaphragm
(48.21) or use a stained specimen, setting the
condenser disc at H (48.14).
Set Koehler illumination (see also page 69):
sharply focus the field diaphragm together with
the specimen by adjusting the condenser in x, y
and z. Swing in the condenser top (48.15).
Set the light ring corresponding to the objective
engraving (e.g. light ring 1 for objective PH 1) on
the condenser disc (48.14).
Fig. 50 Tube and tube lens system with Bertrand lens
1 Interpupillary distance setting of the observation tube,
2 Dial for Bertrand lens (B) or tube lens (1x), 3 Focusing of
Bertrand lens
1
Fig. 51 Auxiliary telescope
1 Adjustable eyelens, 2 Clamp ring for fixing the focus position
2
3
1
2
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