Transmitted Light Polarization - Leica DM R Serie Mode D'emploi

Transmitted light polarization*

See page 65 for objective centration (polarized
light microscopes only).
Adjust the light source, diaphragms and
condenser as for transmitted light brightfield
(page 69); the following description applies for
polarized light microscopes (Fig. 54) and for
other microscopes retrofitted with polarizers
(polarization contrast, Fig. 27).
Crossing the p olarizers
Focus an empty area of the specimen or remove
the specimen from the light path.
Remove any compensators (50.13; 27.6), Bert-
rand lens (54.2 or 50.2) and incident light
reflectors (54.12) from the light path.
Rotate the condenser disc (54.16) to pos. H.
Rotate the objective nosepiece (60.7) to pos. H.
Insert the analyser (54.3) and preadjust as
follows, corresponding to the polarizer used:
Analyser IC/P (30.5)
Make index coincide
exactly, the λ mark must
point downwards
Analyser 360 (30.1)
Set exactly at 90.0° pos.
(DIN standard)
Polarizer Ø 32 mm (27.3)
insert from the right (27.6)
or place on the window
in the microscope base
(27.3) or
Polarizer IC/P (54.17)
IC setting = 90°
(i.e. vibration direction
N – S (54.17 )
Polarizer IC/P (54.17)
0° setting (vibration
direction E – W ↔ )
Looking at the empty field of view, set the
optimum extinction position by rotating the
polarizer (never the analyser!)
Fig. 54 Controls on polarized light microscope
1 Centration* of Bertrand lens, 2 Bertrand lens* on/off,
focusing, 3 Analyser, 4 Objective nosepiece clamp screw,
5 Stage clamp, 6 Centration of PH light rings and ICT prisms,
7 Condenser height adjustment, 8 Polarizer rotation clamp,
9 Aperture diaphragm, 10 Field diaphragm, 11 Tube lens 1x/1.6x*,
12 Quadruple* turret for incident light techniques, 13 Com-
pensator slot (tube slot), 14 45° clickstop (hidden), 15 Stage
rotation clamp, 16 Condenser disc, 17 Index adjustment of
transmitted light polarizer
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