Operation Of Incident Light Fluorescence - Leica DMIL Mode D'emploi

Operation of incident-light fluorescence
Note
For microscopes with integrated incident-light
fluorescence facility only.
For the viewing of transparent objects using
incident-light fluorescence, it is recommended
to make an adjustment with transmitted-light
first.
Fig. 50
1 Light stop, 2 BG 38 filter, 3 Filter block slide
3
1 2
• Open the light stop by moving the lever (50.1).
= Light stop moved out of beam path
= Licht stop moved into beam path
• Slide the filter block into the beam path (50.3).
• Position the specimen and focus. The field
diaphragm is installed and pre-centred so that
no adjustment is necessary.
If a reddish background of the specimen
becomes visible with UV excitation, you can
eliminate this effect by moving the BG 38 red
attenuating filter (50.2) into the beam path.
= Filter moved out of beam path
= Filter moved into beam path
51