Leica BIOSYSTEMS NCL-g-SARC Mode D'emploi page 4

Warnings and Precautions
Novocastra H302: Harmful if
Lyophilised
swallowed.
Antibodies H317: May cause an
Contains a mixture allergic skin reaction.
of: Sodium H334: May cause allergy
Azide (<10%), or asthma symptoms or
Benzylpenicillin breathing difficulties if
Sodium (<10%), inhaled.
Streptomycin H411: Toxic to aquatic
Sulphate (<10%). life with long lasting
Signal words: effects.
Danger. EUH032: Contact with
acids liberates very
toxic gas.
This reagent has been prepared from the supernatant of cell culture. As it is a biological product, reasonable care should be taken when
handling it.
This reagent contains sodium azide. A Material Safety Data Sheet is available upon request or available from www.LeicaBiosystems.com
Consult federal, state or local regulations for disposal of any potentially toxic components.
Specimens, before and after fixation, and all materials exposed to them, should be handled as if capable of transmitting infection and
disposed of with proper precautions.
reagents and specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Seek
medical advice.
Minimize microbial contamination of reagents or an increase in non-specific staining may occur.
Incubation times or temperatures, other than those specified, may give erroneous results. Any such changes must be validated by the
user.
Liquid nitrogen due to its excessively cold temperature causes burns and protective clothing, including gloves and visor, should be used
when handling. Use in a well ventilated area.
Isopentane is highly flammable and harmful by ingestion and inhalation. It is also irritating to skin and eyes, and is a narcotic in high
concentration.
Quality Control
Differences in tissue processing and technical procedures in the user's laboratory may produce significant variability in results,
necessitating regular performance of in-house controls in addition to the following procedures.
Controls should be fresh autopsy/biopsy/surgical specimens frozen as soon as possible in the same manner as the patient sample(s).
Positive Tissue Control
Used to indicate correctly prepared tissues and proper staining techniques.
One positive tissue control should be included for each set of test conditions in each staining run.
A tissue with weak positive staining is more suitable than a tissue with strong positive staining for optimal quality control and to detect
minor levels of reagent degradation.
Recommended positive control tissue is skeletal muscle.
If the positive tissue control fails to demonstrate positive staining, results with the test specimens should be considered invalid.
Negative Tissue Control
Should be examined after the positive tissue control to verify the specificity of the labeling of the target antigen by the primary antibody.
Recommended negative control tissue has not been evaluated.
Alternatively, the variety of different cell types present in most tissue sections frequently offers negative control sites, but this should be
verified by the user.
Non-specific staining, if present, usually has a diffuse appearance. Sporadic staining of connective tissue may also be observed in
sections from excessively formalin-fixed tissues. Use intact cells for interpretation of staining results. Necrotic or degenerated cells often
stain non-specifically. False-positive results may be seen due to non-immunological binding of proteins or substrate reaction products.
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They may also be caused by endogenous enzymes such as pseudoperoxidase (erythrocytes), endogenous peroxidase
(cytochrome C), or endogenous biotin (eg. liver, breast, brain, kidney) depending on the type of immunostain used. To differentiate
endogenous enzyme activity or non-specific binding of enzymes from specific immunoreactivity, additional patient tissues may be stained
exclusively with substrate chromogen or enzyme complexes (avidin-biotin, streptavidin, labeled polymer) and substrate-chromogen,
respectively. If specific staining occurs in the negative tissue control, results with the patient specimens should be considered invalid.
Negative Reagent Control
Use a non-specific negative reagent control in place of the primary antibody with a section of each patient specimen to evaluate
non-specific staining and allow better interpretation of specific staining at the antigen site.
Patient Tissue
Examine patient specimens stained with NCL-g-SARC last. Positive staining intensity should be assessed within the context of any
non-specific background staining of the negative reagent control. As with any immunohistochemical test, a negative result means that
the antigen was not detected, not that the antigen was absent in the cells/tissue assayed. If necessary, use a panel of antibodies to
identify false-negative reactions.
G-SARC-CE
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P261: Avoid breathing dust/fumes/gas/mist/vapours/spray.
P264: Wash hands thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P272: Contaminated work clothing should not be allowed out of the workplace.
P273: Avoid release to the environment.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P284: In case of inadequate ventilation wear respiratory protection.
P301+312: IF SWALLOWED: Call a POISON CENTER/doctor/ if you feel unwell.
P302+352: IF ON SKIN: Wash with plenty of soap and water.
P304+340: IF INHALED: Remove person to fresh air and keep comfortable for
breathing.
P330: Rinse mouth.
P333+313: If skin irritation or rash occurs: Get medical advice/attention.
P342+311: If experiencing respiratory symptoms: Call a POISON CENTER/
doctor.
P362+364: Take off contaminated clothing and wash it before reuse.
P391: Collect spillage.
P501: Dispose of contents/container to hazardous or special waste collection
point.
Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with
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