Leica BIOSYSTEMS NCL-g-SARC Mode D'emploi page 3

Novocastra Lyophilized Mouse Monoclonal Antibody
TM
Gamma-Sarcoglycan
Product Code: NCL-g-SARC
Intended Use
For in vitro diagnostic use.
NCL-g-SARC is intended for the qualitative identification by light microscopy of Gamma-Sarcoglycan by immunohistochemistry. The
clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should
be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Principle of Procedure
Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody
to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate
with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The
specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential
diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
Clone
35DAG/21B5
Immunogen
Synthetic peptide containing amino acids 167–178 of the rabbit gamma-sarcoglycan sequence (Noguchi et al., 1995).
Specificity
Human gamma-sarcoglycan (35 kD).
Reagent Composition
NCL-g-SARC is a lyophilized tissue culture supernatant containing sodium azide as a preservative. The user is required to reconstitute
the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
Ig Class
IgG2b, kappa
Total Protein Concentration
Total Protein
Refer to vial label for lot specific total protein concentration.
Antibody Concentration
Greater than or equal to 22.5 mg/L as determined by ELISA. Refer to vial label for batch specific Ig concentration.
Recommendations On Use
Immunohistochemistry (see Methodology) on frozen sections. Suggested dilution: 1:100 for 60 minutes at 25 C. This is provided as a
o
guide and users should determine their own optimal working dilutions.
Storage and Stability
Store unopened antibody at 2–8 C. Under these conditions, there is no significant loss in product performance up to the expiry date
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indicated on the vial label. Do not use after expiration date indicated on the vial label. The reconstituted antibody is stable for at least two
months when stored at 2–8 C. For long term storage, it is recommended that aliquots of the reconstituted antibody are stored frozen at
o
-20 C (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day
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of use. Return to 2–8 C immediately after use. Storage conditions other than those specified above must be verified by the user.
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Specimen Preparation
Freeze specimen tissue blocks in isopentane chilled in liquid nitrogen (see Warnings and Precautions). The specimens do not require
further fixation but should be embedded in OCT compound (Sakura, Product No. Tissue-Tek 4583).
™
G-SARC-CE
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