3M MDA2LMO96 Mode D'emploi page 9

3. Create or edit a run with data for each sample. Refer to the 3M Molecular Detection System User Manual for details.
Note: The 3M Molecular Detection Instrument must reach and maintain temperature of 60°C before inserting the 3M
Molecular Detection Speed Loader Tray with reaction tubes. This heating step takes approximately 20 minutes and is
indicated by an ORANGE light on the instrument's status bar. When the instrument is ready to start a run, the status bar
will turn GREEN.
Lysis
1. Allow the lysis solution (LS) tubes to warm up by setting the rack at room temperature (20-25°C) overnight
(16-18 hours). Alternatives to equilibrate the LS tubes to room temperature are to set the LS tubes on the laboratory
bench for at least 2 hours, incubate the LS tubes in a 37 ±1°C incubator for 1 hour or place them in a dry double block
heater for 30 seconds at 100°C.
2. Invert the capped tubes to mix. Proceed to next step within 4 hrs.
3. Remove the enrichment broth from the incubator.
4. One LS tube is required for each sample and the Negative Control (NC) (sterile enrichment medium) sample.
4.1 LS tube strips can be cut to desired LS tube number. Select the number of individual LS tubes or 8-tube strips
needed. Place the LS tubes in an empty rack.
4.2 To avoid cross-contamination, decap one LS tubes strip at a time and use a new pipette tip for each transfer step.
4.3 Transfer enriched sample to LS tubes as described below:
Transfer each enriched sample into an individual LS tube first. Transfer the NC last.
4.4 Use the 3M™ Molecular Detection Cap/Decap Tool-Lysis to decap one LS tube strip - one strip at a time.
4.5 Discard the LS tube cap – if lysate will be retained for retest, place the caps into a clean container for re-application
after lysis. For processing of retained lysate, see Appendix A.
4.6 Transfer 20 µL of sample into a LS tube unless otherwise indicated in Protocols from Tables 2, 3, or 4.
5. Repeat step 4.2 until each individual sample has been added to a corresponding LS tube in the strip.
6. Repeat steps 4.1 to 4.6 as needed, for the number of samples to be tested.
7. When all samples have been transferred, transfer 20 µL of NC (sterile enrichment medium, e.g., Demi-Fraser Broth) into
a LS tube. Do not use water as a NC.
8. Verify that the temperature of the 3M Molecular Detection Heat Block Insert is at 100 ±1°C.
9. Place the uncovered rack of LS tubes in the 3M Molecular Detection Heat Block Insert and heat for 15 ±1 minutes.
During heating, the LS solution will change from pink (cool) to yellow (hot).
Samples that have not been properly heat treated during the assay lysis step may be considered a potential biohazard
and should NOT be inserted into the 3M Molecular Detection Instrument.
10. Remove the uncovered rack of LS tubes from the heating block and allow to cool in the 3M Molecular Detection Chill
Block Insert at least 5 minutes and a maximum of 10 minutes. The 3M Molecular Chill Block Insert, used at ambient
temperature without the Molecular Detection Chill Block Tray should sit directly on the laboratory bench. When cool,
the lysis solution will revert to a pink color.
11. Remove the rack of LS tubes from the 3M Molecular Detection Chill Block Insert.
00:15:00
99-101ºC
00:05:00
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20 µl
20-25ºC