Leica BIOSYSTEMS BOND Ready-to-Use ISH Lambda Probe PB0669 Mode D'emploi page 3

BOND Ready-to-Use ISH
Lambda Probe
Catalog No: PB0669
Intended Use
This reagent is for in vitro diagnostic use.
Lambda Probe is intended for use in the qualitative identification of Lambda light chain messenger RNA (mRNA) in formalin-fixed,
paraffin-embedded tissue by in situ hybridization (ISH) using the automated BOND system (includes Leica BOND-MAX system and
Leica BOND-III system).
The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and
should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Summary and Explanation
Immunoglobulins are glycoproteins produced in mature B-cells against a specific antigen. Each individual immunoglobulin molecule
comprises two heavy and two light polypeptide chains. There are five classes of immunoglobulin, determined by the type of heavy chain.
In contrast, there are only two types of light chain: Kappa or Lambda. Each individual immunogloblin molecule is composed of one of five
classes of heavy chains and either Kappa or Lambda light chains. In normal human lymphoid populations, the ratio of Kappa to Lambda
light chains is approximately 2:1
B-cell neoplasms are thought to arise from a single transformed cell (monoclonal)
proliferation of a number of B-cells (polyclonal). Since immunoglobulins from the same B-cell contain either Kappa or Lambda light
chains, light chain restriction or monoclonality can be used to make the distinction between reactive and neoplastic B-cell proliferations
Lambda Probe is used in conjunction with Kappa Probe for the detection of antibody producing B-cells in formalin-fixed, paraffin-
embedded tissue. The method is reproducible and should result in intense, brown cytoplasmic staining of cells containing Lambda light
chain mRNA.
Reagents Provided
Lambda Probe is a fluorescein-conjugated oligonucleotide probe supplied in hybridization solution.
Total volume = 5.5 mL
Dilution and Mixing
Lambda Probe is ready to use. Reconstitution, mixing, dilution or titration of this reagent is not required.
Materials Needed but not Provided
Refer to "Using BOND Reagents" in your BOND user documentation for a complete list of materials required for specimen treatment and
in situ hybridization staining using the BOND system (includes Leica BOND-MAX system and Leica BOND-III system).
Storage and Stability
Store at 2–8 C. The product is stable under these conditions up to the expiry date indicated on the container label.
o
There are no obvious signs indicating contamination and/or instability. Appropriate positive and negative tissue controls should be run at
the same time as test tissue.
Return to 2–8 °C immediately after use.
Storage conditions other than those specified above must be verified by the user
Precautions
• This product is intended for in vitro diagnostic use.
LAMBDA PROBE
Contains Formamide (<50%)
and Dextran Sulphate
(<30%).
GHS07: Exclamation mark.
GHS08: Health hazard.
Signal words: Danger.
• To obtain a copy of the Material Safety Data Sheet contact your local distributor or regional office of Leica Biosystems, or alternatively,
visit the Leica Biosystems' Web site, www.LeicaBiosystems.com.
• Specimens, before and after fixation, and all materials exposed to them, should be handled as if capable of transmitting infection and
disposed of with proper precautions
reagents or specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Seek
medical advice.
• Consult Federal, State or local regulations for disposal of any potentially toxic components.
.
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H360D: May damage
the unborn child.
H315: Causes skin
irritation.
H319: Causes serious
eye irritation.
. Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with
5
. In contrast, benign or reactive states result in
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.
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P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and
understood.
P260: Do not breathe dust/fumes/gas/mist/vapours/spray.
P308+313: IF exposed or concerned: Get medical advice/attention.
P314: Get medical advice/attention if you feel unwell.
P264: Wash hands thoroughly after handling.
P280: Wear protective gloves/protective clothing/eye protection/face
protection.
P305+351+338: IF IN EYES: Rinse cautiously with water for several
minutes. Remove contact lenses, if present and easy to do. Continue
rinsing.
P337+313: If eye irritation persists: Get medical advice/attention.
P302+352: IF ON SKIN: Wash with plenty of soap and water.
P332+313: If skin irritation occurs: Get medical advice/attention.
P362+P364: Take off contaminated clothing and wash it before reuse.
Restricted to professional users.
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PB0669
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