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Setting the Incubation Well Configuration
Incubation wells 1-5 and 6-10 can be configured to run the same or different types of
3M™ Attest™ Rapid Readout Biological Indicators. The flashing "A" in Attest indicates the
setting may be changed by the user. There are three incubation well configuration options:
LCD Panel Configuration Setting
Attest 1291
Attest 1291
Attest 1292
Use the Up Arrow or Down Arrow buttons to move through the three options until the
desired configuration setting is displayed.
Press
to select the desired option and advance to the Accept screen.
Note 1: If there are any incubating BIs which do not yet have a final result (+ or -)
displayed, the well configuration for the occupied wells will NOT change until the final
result is displayed on the LCD panel and the BI is removed from the reader.
Note 2: Ensure the color-coded incubation well configuration sticker surrounding the
incubation wells matches the programmed well configuration.
Accept Screen
Use the up or down buttons to toggle between Yes and No, press
(Yes selected) or retain current settings (No selected) and exit the configuration mode. The
LCD panel will return to the standard display.
INSTRUCTIONS FOR USE - Readout Procedure
Instructions for Biological Indicator (BI) Activation and Incubation
WARNING: Match the cap color of the 3M™ Attest™ Rapid Readout Biological
Indicator with the color-coded configuration sticker surrounding the 3M™ Attest™
Auto-reader 390 incubation wells.
CAUTION: Wear safety glasses when activating 3M™ Attest™ Rapid Readout
Biological Indicators.
CAUTION: Allow the 3M™ Attest™ Rapid Readout Biological Indicator to cool
for the recommended time period before activating. Activating or excessive handling of
the biological indicator before cooling may cause the glass ampoule to burst.
CAUTION: To avoid the possibility of the 3M™ Attest™ Rapid Readout
Biological Indicator vial absorbing fluorescent residue from a chemical indicator or
tape, place the 3M™ Attest™ Rapid Readout Biological Indicator vial so it does not
come in direct contact with chemical indicators or tape.
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Result
Auto-reader wells 1 - 5 configured for
1291 BIs (blue cap, 1 hour readout) and wells
Attest 1292
6 - 10 configured for 1292 BIs (brown cap,
3 hour readout)
Auto-reader wells 1 –10 configured for
Attest 1291
1291 BIs (blue cap, 1 hour readout)
Auto-reader wells 1 –10 configured for
Attest 1292
1292 BIs (brown cap, 3 hour readout)
to accept changes
Follow this procedure for every 3M™ Attest™ Rapid Readout Biological Indicator to
be read.
While wearing safety glasses, CLOSE the cap of
the cooled 3M™ Attest™ Rapid Readout Biological
Indicator (BI) by pushing cap down.
CRUSH the glass media ampoule in the Crusher Well
built into the top of the 390 Auto-reader.
Holding BI by the cap, TAP the bottom of the vial on a
tabletop until media wets spore strip at bottom of vial.
Do not tap BI on Auto-reader.
PLACE the crushed BI in color-coded incubation well.
Observe 3M™ Attest™ Auto-reader 390 LCD panel to
confirm correct placement of the BI. If the BI is correctly
seated, the 3M™ Attest™ Auto-reader 390 LCD panel
will display ***, **, * and then remaining minutes of
incubation directly below the incubating BI.
RECORD BI result.
WARNING: Do not remove the 3M™ Attest™ Rapid Readout Biological
Indicator from the incubation well until the (+) or (-) symbol on the LCD panel indicates
the test is complete.
INSTRUCTIONS FOR USE – Additional Information
BI Placement
Wells must be configured and color coded to match the cap of the 3M™ Attest™
Rapid Readout Biological Indicator, e.g. the 3M™ Attest™ 1291 Rapid Readout
Biological Indicator with a blue cap goes into a blue well and the 3M™ Attest™
1292 Rapid Readout Biological Indicator with a brown cap goes into a brown well.
Remaining incubation time, in minutes, will be displayed on the LCD display to indicate
the 3M™ Attest™ Rapid Readout Biological Indicator is incubating in the reader well and
should not be touched. The time remaining display indicates the results are not available
and incubation is in progress.
Do not remove 3M™ Attest™ Rapid Readout Biological Indicator until the (+) or (-)
symbol on the LCD panel indicates the test is complete.
Note: If 3M™ Attest™ Rapid Readout Biological Indicator is inadvertently removed
before the incubation period is complete, an alarm will sound and a C2 Caution code will
be displayed on the LCD panel under that well. You must return the 3M™ Attest™
Rapid Readout Biological Indicator to the reader well within 10 seconds or the
results will be lost.
WARNING: Do not remove or change placement of 3M™ Attest™ Rapid
Readout Biological Indicator once it is placed into a well. Doing so may result in loss of
results and the 3M™ Attest™ Rapid Readout Biological Indicator test may be invalid.
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