Integrated Phase Contrast (Tl) - Leica Microsystems DMI3000 B Mode D'emploi

8.2.6 Integrated Phase Contrast (TL)

Leica DMI3000 B:
• If necessary, adjust the TL bright fi eld position
at the fi lter slider.
• Select a bright fi eld objective with eyepoint B
or C.
• Select the appropriate light ring at the con-
denser (see table).
• Open the aperture of the condenser complete-
ly.
• Remove all other optical components such
as analyzers, polarizers or IC prisms from the
beam path.
• Slide the phase contrast front module to the
correct eyepoint, B or C.
• Insert a phase contrast specimen.
• Set the brightness at the light potentiometer
• Focus the image with the focus wheels.
IP0 for 5x,
IP1 for 10x,
for 20x,
IP2 for 40x,
IP3 for 63x,
e.g. NPlan 5x
e.g. NPlan 10 x
e.g. NPlan L 20 x
e.g. HCX PL FL L 40 x
e.g. PL FL 63x/0.70
Leica DMI4000 B and Leica DMI6000 B:
• Use the TL/IL function button to switch to
transmitted light (TL).
• Select the IPC contrast method (integrated
phase contrast). by pressing the variable but-
ton IPH. Alternatively: press the variable button
CHANGE TL . (For details on button assign-
ments, please see the identifi cation sheet.)
PH will appear on the LeicaScreen. Motor-
ized condensers will now switch to the correct
light ring. Coded condensers must be switched
manually.
• Insert a transmitted light specimen.
• Select a suitable objective (eyepoint B or C).
• Slide the phase contrast front module to the
correct eyepoint, B or C.
• Focus the image with the knob on the Smart-
Move or the focusing wheel and adjust the in-
tensity with the INT function buttons.
Note:
When selecting the phase contrast method, the
aperture diaphragm is opened fully and can not
be adjusted.
objective with eyepoint B
objective with eyepoint B and
objective with eyepoint C
objective with eyepoint C
objective with eyepoint C
8. Operation
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