Phase Contrast (Tl) - Leica Microsystems DMI3000 B Mode D'emploi

8.2.2

Phase Contrast (TL)

(integrated phase contrast, see 8.2.6)
Leica DMI3000 B:
• If necessary, adjust the TL bright fi eld position
at the fi lter slider.
• Select a phase contrast objective.
• Select the suitable light ring on the condenser.
• Open the aperture of the condenser com-
pletely.
• Remove all other optical components such
as analyzers, polarizers or IC prisms from the
beam path.
• Insert a phase contrast specimen.
• Set the brightness at the light potentiometer
• Focus the image with the focus wheels.
Leica DMI4000 B and Leica DMI6000 B:
• Use the TL/IL function button to switch to
transmitted light (TL).
• Select the PH (phase contrast) contrast meth-
od
by pressing the variable button PH.
Alternatively: press the variable button
CHANGE TL .
(For details on button assignments, please see
the identifi cation sheet.)
PH will appear on the LeicaScreen.
Motorized condensers will now switch to the
correct light ring. Coded condensers must be
switched manually.
• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
Objectives that are suitable for phase contrast
are engraved with PH.
• Focus the image with the knob on the Smart-
Move or the focusing wheel and adjust the in-
tensity with the INT function buttons.
Note:
When selecting the phase contrast method, the
aperture diaphragm is opened fully and can not
be adjusted.
8. Operation
85