Contrast Methods; Bright Field (Tl) - Leica Microsystems DMI3000 B Mode D'emploi

8.2 Contrast Methods

All of the contrast methods of the Leica DMI4000
B and Leica DMI6000 B can be selected and con-
trolled via the variable function buttons and the
Leica Application Suite (LAS). The only excep-
tions are methods that involve com ponents re-
quiring manual control (e.g. systems with manual
analyzers). The following section describes the
use of the function buttons on the stand. For in-
structions on the use of the software, please re-
fer to the separate manual.
Contrast methods for the Leica DMI3000 B are
controlled via the manual condenser, the manual
objective turret, as well as turning knobs and
sliders at the microscope.
8.2.1

Bright Field (TL)

Leica DMI3000 B:
• If necessary, adjust the TL bright fi eld position
at the fi lter slider.
• Set the condenser to the bright fi eld position.
• Remove all other optical components such
as analyzers, polarizers or IC prisms from the
beam path.
• Insert a transmitted light specimen.
• Select your objective
• Set the brightness at the light potentiometer
• Focus the image with the focus wheels.
Leica DMI4000 B and Leica DMI6000B:
Note:
If all positions of the fi lter turret are occupied, fi l-
ter cube „A" can be swapped for fi lter cube „A-
TL" using the Leica Application Suite (LAS/LAS
AF). TL contrast methods are possible with that
fi lter cube.
8. Operation
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