Differential Interference Contrast (Tl) - Leica Microsystems DMI3000 B Mode D'emploi

8. Operation

8.2.5 Differential Interference Contrast (TL)

Leica DMI3000 B:
• If necessary, adjust the TL bright fi eld position
at the fi lter slider.
• Select an objective.
• At the condenser, select the appropriate Wol-
laston prism condenser.
• At the objective turret, select the appropriate
Wollaston prism objective.
• Move the polarizer on the condenser into the
beam path.
• Insert the analyzer into the right side of the
stand until it clicks into position.
• Insert a specimen.
• Set the brightness at the light potentiometer
• Focus the image with the focus wheels.
• Use the knurled wheel below the objective tur-
ret for fi ne adjustment (Fig. 111).
Leica DMI4000 B and Leica DMI6000 B:
• Use the TL/IL function button to switch to
transmitted light (TL).
• Select the DIC contrast method
by pressing the variable button DIC.
Alternatively: press the variable button
CHANGE TL .
(For details on button assignments, please see
the identifi cation sheet.)
DIC will appear on the LeicaScreen.
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• The polarizer in the condenser and the suitable
condenser prism are automatically positioned
in the beam path. The corresponding objec-
tive prism and the analyzer cube are also posi-
tioned automatically.
• Place a DIC specimen on the stage.
• Rotate an appropriate objective into place.
• Focus the image with the knob on the Smart-
Move or the focusing wheel and adjust the in-
tensity with the INT function buttons.
• Use the knurled wheel below the objective tur-
ret for fi ne adjustment (Fig. 111).
Manual alternative:
• Move the polarizer on the condenser into the
beam path manually.
• Insert the analyzer manually into the right side
of the stand until it clicks into position (Fig. 110).
Adjust the objective and condenser prisms
manually until a valid combination appears on
the display.
• Use the knurled wheel below the objective tur-
ret for fi ne adjustment (Fig. 111).
Fig. 111 DIC disk with knurled wheel for fi ne adjustment