Leica Microsystems DMI3000 B Mode D'emploi page 74

7. Start-up
Leica DMI3000 B:
• If necessary, adjust the TL bright fi eld position
at the fi lter slider.
• Set the condenser to the bright fi eld position.
Leica DMI4000 B and Leica DMI6000 B:
• Press the BF (bright fi eld) button (one of the
variable function buttons on the stand).
• Instead of an eyepiece, place a focusing tel-
escope (Fig. 93) in the observation tube or
ac tivate the Bertrand lens (pull rod (94.1) on
tube).
• Select the phase contrast objective with the
lowest magnifi cation.
• Focus on the specimen.
• Focus the ring structure (95a) by loosening
the clamping ring (93.2) somewhat and moving
the eyelens (93.1), or focus the Bertrand lens
(94.2).
• Retighten the clamping ring.
Fig. 93 Focusing telescope
1 Adjustable eyelens
2 Clamping ring for fi xing the focus position
74
Leica DMI3000 B:
• Select the light ring for the active objective on
Leica DMI4000 B and Leica DMI6000 B:
• Press the PH (phase contrast) button (one of
Fig. 94
1
2
Fig. 95
PH=phase contrast ring, LR=light ring
a Condenser in bright fi eld (BF) position
b Condenser in phase contrast (PH) position
1
c Light ring and phase ring centered
2
the condenser.
the variable function buttons behind the focus
wheels). The light ring will be selected in the
condenser.
Activating the Bertrand lens
Focusing the Bertrand lens
1
Phase contrast centering procedure
Light ring (LR) not centered
a b
PH
2
c
LR