Combining Functions; Performing Washing And Suspension Procedure - Hettich ROTOLAVIT Mode D'emploi

EN
15.2

Combining functions

Through the careful operation of the centrifuge the user determines the precision of the test results.
If the permissible weight difference within the rotor loading has been exceeded, the drive shuts down during
the start-up, the unbalance display lights up, and IMBALANCE is displayed.
A function can be terminated at any time by pressing the
If OPEN LID or WASH COMPLETE or SUSP. COMPLETE or man. SUSP. DONE are displayed, then the
further operation of the centrifuge is only possible after a single opening of the lid.
Be sure that the system is filled with physiological saline solution before working with test material.
In order not to damage the rotor a washing procedure may only be started with the
centrifugation run may only be started with the
• Turn on the mains switch. Switch position Ι.
15.2.1

Performing washing and suspension procedure

•
Load the rotor and close the centrifuge lid.
•
Set the parameters or call up a programme (see the "Programming" chapter).
•
Press the button. The rotation indicator
START WASH
The washing procedure and the production of a suspension are executed:
1. Filling in the physiological saline solution.
The LED in the
SALINE
PROG CYC ml RPM
1 3 3 1100
If the program parameter SalWash/ml has the value "0.0", then saline solution is not filled.
2. Sedimentation.
The time only begins to count after the set rotation speed is reached.
Briefly SPIN is displayed.
PROG CYC ml RPM
1 3 3 3500
If the program parameter Twash(min) and Twash(sec) have the value "0:00", then a
centrifugation run is not performed.
3. Decanting.
DCNT is displayed.
PROG CYC ml RPM
1 3 3 425 DCNT
If the program parameters Twash(min) and Twash(sec) have the value "0:00", then decanting is
not performed.
4. Centrifugation run to centrifuge the remaining droplets on the side of the tube to the bottom of the tube.
DOWN is displayed.
PROG CYC ml RPM
1 3 3 2000 DOWN
The centrifugation run is only performed if the function is activated (global parameter
D.SpinDown (s) > "0" and program parameter D.SpinDown = "ON").
52/130
SPIN
lights up as long as the rotor is turning.
key illuminates whilst the saline solution is being pumped in and FILL is displayed.
t/ min:s
FILL
t/ min:s
SPIN
t/ min:s
t/ min:s
key.
STOP
key only after the rotor has come to a standstill.
key or a
START WASH