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Parameter
Minimum sample
volume
Scatter resolution
Sample flow rate
Maximal event rate
Lasers
Fluorescence sensitivity
(MESF)
Absolute cell count
performance
Sample carryover
Emission detectors
Table 9.2: Operation details of the MACSQuant X
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Specification
1 μL (20 µL recommended for volumetric counting applications)
Scatter performance is optimized for resolving human peripheral blood lymphocytes,
monocytes, and granulocytes
25-100 μl/min plus automated flow rate to maintain 500, 1000, or 2000 events/second
Up to 15,000 events/second
405 nm, 40 mW
488 nm, 30 mW
640 nm, 20 mW
FITC < 200; PE < 100; APC < 150
Accuracy +/- 7%; reproducibility (CV) <5%
≤ 0.1% in Standard mode
Channel
FSC
SSC
V1
V2
B1
B2
B3
B4
R1
R2
Filter
488/10 nm
488/10 nm
450/50 nm
525/50 nm
525/50 nm
585/40 nm
655-730 nm
750 nm LP
655-730 nm
750 nm LP
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