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Troubleshooting
8.1
Syringe pump leakage
Correct overnight and long-term storage assures that no salt deposits accumulate in the syringe pump, which
could cause wear of the pump seal. In case of leakage of the syringe pump, turn off the instrument and check
the connection between the syringe pump and the valve. If it is loose, tighten it counterclockwise. If the
leakage persists, contact Miltenyi Biotec Technical Support to replace either the pump syringe or the pump
seal.
8.2
Air bubbles during measurement/no event acquisition
Check for leakage within the fluidics and tubing system and perform a rinse program. Also, ensure that the
sheath particle filter is devoid of air. Ensure that the needle calibration was performed correctly. Furthermore,
check that the tubes connected to the bottle closures are tightly sealed. If the problem persists, contact
Miltenyi Biotec Technical Support.
8.3
Excessive debris is present during acquisition
1 Check all fluidics to ensure that all tubes are connected properly.
2 Ensure that the sheath particle filter is devoid of air.
3 Click the Rinse button in the Instrument status bar. Perform this twice.
4 If this does not work, try to clean the system by using the Clean and/or Flush program.
5 To access the Clean program, right-click on the Rinse button. Select Clean to perform a system clean with
1% hypochlorite solution. Follow the instructions in the dialog box that appears and place the required
solution in the Single tube rack. The Single tube rack must be attached and selected in the Experiment tab
in order to perform a Clean program. The program takes approximately 10 minutes.
6 To start a system backflush, right-click on the Rinse button and select Flush.
8.4
Touchscreen remains dark
If the touchscreen remains dark, check if the power cord is plugged in and the power supply is switched on. If
the instrument is switched on and the touchscreen remains dark, switch off the instrument, wait five seconds
and switch on again. If the instrument still does not initialize, contact Miltenyi Biotec Technical Support.
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