CooperSurgical Wallace DNS1633-950 Notice D'utilisation page 3

Instructions for use
1
Oocyte recovery should be performed under either general anaesthetic or
analgesia.
2
Place the patient in the lithotomy position, prepare the vulva and vagina for
full aseptic technique and cover the area in sterile drapes.
3
Cover the ultrasound transducer with a sterile sleeve with a small amount of
coupling gel covering the end of the probe within the sleeve. Introduce into
the vagina and scan the pelvis to establish the position of the ovary, the
development of the endometrium and the number and position of follicles
to be aspirated.
4
Remove the Oocyte Recovery System from its packaging and connect a
syringe or vacuum pump to the Luer compatible connector on the vacuum
tube (use the pump adaptor if required) and a sterile collection tube to the
silicone bung.
5
Connect a sterile syringe or other suitable delivery system containing
flushing media to the Luer compatible connector at the end of the flushing
tube.
6
Flush the transfer and flushing tubes/lumen with media. After flushing
purge the transfer system of fluid and empty or discard the collection tube.
Prime the flushing tube and flushing lumen with media.
WARNING
Test the system to ensure all connections are sound and flow can be
achieved. Failure to test the system may result in failure to obtain the
required sample and/or tissue damage.
7
Insert the needle into the needle guide of the ultrasound transducer,
ensuring that there is smooth movement along its length.
8
Insert the ultrasound transducer and needle into the vagina and direct the
probe towards the posterolateral aspect of the vaginal fornix. Manipulate
the probe until the follicles appear in close proximity to the needle.
WARNING
Failure to use ultrasound to locate the follicles or incorrect needle
guidance may result in failure to obtain the required sample and/or
tissue damage.
9
Advance the needle into the nearest follicle. If the ovaries are mobile apply
pressure suprapubically and enter the follicles. When the tip of the needle
can be seen in the follicle, aspirate the contents using either the syringe or
vacuum pump attached to the set. Gentle manipulation of the follicle may
assist separation of oocytes from the follicle wall. An initial vacuum pump
setting in the range 100-200mmHg is suggested, the vacuum source should
be controlled to give the desired flow rate for each patient.
10 If flushing is required, inject media into the follicle using either the syringe
or delivery system connected to the flushing tube, until the follicle returns
to its original size. Aspiration of the follicle should then be repeated.
11 Repeat for all follicles within the ovary. To minimize trauma and possible
bleeding keep the needle tip within the ovary between aspirations.
12 Remove the collection tube from the silicone bung when full (do not allow
the collection tube to become so full that oocytes are aspirated into the
vacuum source). Replace with a new sterile collection tube.
13 When all the follicles in one ovary have been aspirated, repeat the
procedure for the other ovary. If necessary, once all accessible follicles have
been emptied, aspirate any blood or fluid that is present within the pouch
of Douglas.
14 Withdraw the transducer and needle, check the vagina for signs of bleeding
and allow the patient to rest. If vaginal bleeding is experienced post
collection, apply steady pressure with a sponge and forceps or use an
absorbable suture if bleeding persists.
15 Dispose of the Oocyte Recovery System in accordance with local medical
hazardous waste practices.
The above are only suggested instructions for use. The final decision on
the technique used is the responsibility of the clinician in charge.
Store at room temperature. Avoid prolonged exposure to elevated
Wallace® is a registered trademark of CooperSurgical, Inc. and its subsidiaries.
CooperSurgical is a registered trademark of CooperSurgical, Inc.
© 2020 CooperSurgical, Inc. All rights reserved.
temperatures.
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