Agilent Technologies Agilent BioHPLC Guide D'utilisation page 5

Other Operating Tips
• Columns should not be maintained at elevated pH or elevated
temperature when not in use (see Table 2).
• New columns may contain a mixture of organic solvents and water,
which may contain buffer salts. See Table 3 for details of the shipping
solvents. Initially, care should be taken not to pass any mobile phase
through the column that may cause a precipitate to form or may not
be fully miscible.
• Agilent BioHPLC and AdvanceBio columns are compatible with all
common eluents used for analysis of biomolecules.
Table 1. Maximum operating parameters – columns
up to 4.6 mm
Column
AdvanceBio Peptide Mapping
AdvanceBio RP-mAb
AdvanceBio Oligonucleotide
ZORBAX RRHD
Poroshell 300
ZORBAX 300SB
PLRP-S 100Å, 300Å
PLRP-S 1000Å, 4000Å
(continued)
Particle size
2.7 µm
3.5 µm
2.7 µm
1.8 µm
5 µm
3.5 µm, 5 µm, 7 µm
3 µm, 5 µm, 8 µm, 10 µm,
10-15 µm, 15-20 µm
5 µm, 8 µm, 10 µm, 30 µm
ENGLISH
Pressure limit
600 bar
600 bar
600 bar
1200 bar
400 bar
400 bar
400 bar
207 bar