Agilent Technologies Agilent BioHPLC Guide D'utilisation page 6

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Table 2. Column operating parameters – pH and temperature
Column
AdvanceBio Peptide Mapping
AdvanceBio RP-mAb
AdvanceBio Oligonucleotide
ZORBAX RRHD
Poroshell 300
Poroshell 300 Extend-C18
ZORBAX 300SB-C18
ZORBAX 300SB-C8, C3, CN
PLRP-S
Note: All silica-based packings have some solubility in pH >6 aqueous mobile phases. When using silica-
based columns at pH >6, best column lifetime is obtained at lower temperatures (40 °C max) using low buffer
concentrations in the range of 0.01 to 0.02 M.
Table 3. Shipping solvents
Column
AdvanceBio Peptide
Mapping
AdvanceBio RP-mAb
AdvanceBio
Oligonucleotide
ZORBAX RRHD
Poroshell 300 and
300Extend
ZORBAX 300SB
PLRP-S
Recommended
pH range
2.0-8.0
1.0-8.0
3.0-11.0
1.0-8.0
1.0-8.0
2.0-11.0
1.0-8.0
1.0-8.0
1.0-14.0
Shipping solvent
Mixture of acetonitrile
and water
Mixture of acetonitrile
and water
Mixture of acetonitrile
and water
Mixture of acetonitrile
and water
Mixture of methanol
and water
Mixture of acetonitrile
and water
7:1 acetonitrile/water
Maximum operating
temperature
60 °C
90 °C
60 °C
80 °C
90 °C
60 °C below pH 8,
40 °C above pH 8
90 °C
80 °C
200 °C
Compatibility
Water and all common organic solvents.
Water and all organic solvents,
including N,N-dimethylformamide
and dimethylsulfoxide.
Water and all common organic solvents.
Modifiers including HFIP and TEAA.
Water and all common organic solvents.
Water and all organic solvents,
including N,N-dimethylformamide
and dimethylsulfoxide.
Water and all common organic solvents.
Aqueous organic solvents
including N,N-dimethylformide and
dimethylsulfoxide. 100% aqueous is not
recommended as it will reduce column
performance and lifetime.