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Remove the oocyte recovery set from its packaging and insert the needle into
the needle guide of the ultrasound transducer, ensuring that there is smooth
movement along its length.
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Attach a syringe or vacuum pump to the Luer compatible connector on the
vacuum line and a sterile collection tube to the silicone bung. The transfer
tube can be pulled through the bung so that it reaches the bottom of the
collection tube, preventing damage to the zona pellucida of the aspirated
oocytes.
WARNING:
Test the system to ensure all connections are sound and flow can be
achieved. Failure to test the system may result in failure to obtain the
required sample and/or tissue damage.
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Insert the ultrasound transducer and needle into the vagina and direct the
probe towards the posterolateral aspect of the vaginal fornix. Manipulate the
probe until the follicles appear in close proximity to the needle.
WARNING:
Failure to use ultrasound to locate the follicles may result in failure to
obtain the required sample and/or tissue damage.
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Advance the needle into the nearest follicle. If the ovaries are mobile apply
pressure suprapubically and enter the follicles with a stabbing movement.
When the tip of the needle can be seen in the follicle, aspirate the contents
until empty using either the syringe or vacuum pump attached to the set.
Gentle manipulation of the follicle may assist separation of oocytes from the
follicle wall.
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If flushing is required, the bung should be removed from the collection
tube. A syringe containing flushing medium can be connected to the Luer
connector, and flushing of the follicle performed. Upon completion of the
flushing, the syringe should be removed and the bung reattached to the
collection tube. Aspiration of the follicle should then be repeated.
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Repeat for all follicles within the ovary. To minimise trauma and possible
bleeding keep the needle tip within the ovary between aspirations.
10 Remove the collection tube from the silicone bung when full. Replace with a
new sterile collection tube.
11 When all the follicles in one ovary have been aspirated, repeat the procedure
with the other ovary. When all accessible follicles have been emptied, aspirate
any blood or fluid that is present within the pouch of Douglas.
12 Withdraw the transducer and needle, check the vagina for signs of bleeding
and allow the patient to rest. If vaginal bleeding is experienced post
collection, apply steady pressure with a sponge and forceps or use an
absorbable suture if bleeding persists.
13 Dispose of the needle set in accordance with local medical hazardous waste
practices.
The above are only suggested instructions for use. The final decision on the
technique used is the responsibility of the clinician in charge.
Wallace, Wallace design mark and Smiths Medical design mark are trademarks
of Smiths Medical. The symbol ® indicates the trademark is registered in the U.S.
Patent and Trademark Office and certain other countries. All other names and marks
mentioned are the trade names, trademarks or service marks of their respective owners.
© 2015 Smiths Medical. All rights reserved.
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