Leica BIOSYSTEMS Novocastra Mode D'emploi page 3

Novocastra Liquid Mouse Monoclonal Antibody
Cytokeratin (8/18)
Product Code: NCL-L-5D3
Intended Use
For in vitro diagnostic use.
NCL-L-5D3 is intended for the qualitative identification by light microscopy of Cytokeratin (8/18) molecules in paraffin sections. The
clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should
be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Principle of Procedure
Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody
to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate
with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The
specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential
diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
Clone
5D3
Immunogen
Cytokeratins from the human breast carcinoma cell line MCF-7.
Specificity
Human cytokeratin 8 and 18 intermediate filaments.
Reagent Composition
NCL-L-5D3 is a liquid tissue culture supernatant containing sodium azide as a preservative.
Ig class
IgG1
Total Protein Concentration
Total Protein
Refer to vial label for lot specific total protein concentration.
Antibody Concentration
Greater than or equal to 420 mg/L as determined by ELISA. Refer to vial label for batch specific Ig concentration.
Recommendations On Use
Immunohistochemistry on paraffin sections.
Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra Enzyme Proteinase K (IHC).
Suggested dilution: 1:200 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working
dilutions.
Visualization: Please follow the instructions for use in the Novolink Polymer Detection Systems. For further product information or
support, contact your local distributor or regional office of Leica Biosystems, or alternatively, visit the Leica Biosystems Web site,
www.LeicaBiosystems.com
The performance of this antibody should be validated when utilized with other manual staining systems or automated platforms.
Storage and Stability
Store at 2–8 C. Do not freeze. Return to 2–8 C immediately after use. Do not use after expiration date indicated on the vial label.
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Storage conditions other than those specified above must be verified by the user.
Specimen Preparation
The recommended fixative is 10% neutral-buffered formalin for paraffin-embedded tissue sections.
Warnings and Precautions
This reagent has been prepared from the supernatant of cell culture. As it is a biological product, reasonable care should be taken when
handling it.
This reagent contains sodium azide. A Material Safety Data Sheet (MSDS) is available upon request for sodium azide.
Consult federal, state or local regulations for disposal of any potentially toxic components.
Specimens, before and after fixation, and all materials exposed to them, should be handled as if capable of transmitting infection and
disposed of with proper precautions. Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with
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reagents and specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Seek
medical advice.
Minimize microbial contamination of reagents or an increase in non-specific staining may occur.
Incubation times or temperatures, other than those specified, may give erroneous results. Any such changes must be validated by the
user.
5D3-L-CE
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