Leica BIOSYSTEMS Kreatech FISH probes FGFR1 (8p11) – XL Mode D'emploi page 9

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Refer to "Reagent Management" in your BOND user documentation
for information about registering new reagents on the BOND system
(includes BOND-MAX system and BOND-III system).
Instructions for use: Pretreatment and Protocols
Kreatech FISH probes have been validated using the BOND FISH kit,
BOND ancillary reagents and the BOND pretreatment and staining
protocols shown in Table 2. The final protocol may vary according
to the tissue used by the user. Any deviations from the prescribed
protocols must be validated by the user.
Table 2: Protocols for running Kreatech FISH probes
BOND Protocol Section
Staining – ISH
Detection
Prestaining –
Preparation
Prestaining – Heat
Pretreatment
Prestaining – Enzyme
Pretreatment
Prestaining – ISH
denaturation
Prestaining - ISH
detection
(1) Not a predefined protocol. For creating the 25 min HIER protocol refer to "Proto-
cols" in your BOND user documentation.
(2) Enzyme 5 is derived from Enzyme Concentrate 1 available in the BOND Enzyme
Pretreatment Kit (AR9551). See Troubleshooting section below for specifics.
Protocol Name
*FISH Protocol D
*Dewax
HIER 25 min with
ER2 (100)
(1)
*Enzyme 5 for 25 min
*D10
*ISH Hybridization
(12Hr)
Kreatech FISH probes
Protocol Type
Post Hybridization
Wash
Removal of paraffin
Heat pretreatment,
Epitope retrieval
Enzymatic digestion
(2)
Denaturation 10 min.
Hybridization 12 hr.