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Contents
1.
Important Notes about this Manual ......
2.
Intended Purpose of Microscopes ........
3.
Safety Notes ...............................................
3.1 General Safety Notes ...............................
3.2 Electrical Safety ........................................
4.
Overview of the Instrument .................... 10
5.
Unpacking the Microscope .................... 18
6.
Assembling the Microscope .................. 20
6.1 Stage ............................................................ 20
6.2 Condenser ................................................... 22
6.3 Tube and Eyepieces .................................. 23
6.4 Objectives ................................................... 23
6.5 Light Source - Transmitted Light Axis ... 24
6.6 Components for Fluorescence
Applications ................................................ 26
6.6.1 Fluorescence Illuminator .............. 26
6.6.2 106 z Lamp Housing ........................ 26
6.6.3 Equipping the
Fluorescence Turret Disk .............. 29
6.7 Analyzer and Polarizer ............................ 30
6.8 Lambda Plate Compensator .................... 31
6.9 DIC Prisms .................................................. 31
6.10 Optional Accessories ............................... 31
6.11 Connection to the Power Supply ............ 33
7.
Start-up ........................................................ 34
7.1 Switching on the Microscope ................. 34
7.2 Köhler Illumination .................................... 34
7.3 Checking Phase Contrast Rings ............. 36
7.4 Adjustment of Condenser Prisms ........... 37
7.5 Adjusting the Light Sources .................... 38
8.
Operation .................................................... 43
8.1 Switching on ............................................... 43
8.2 Stages and Object Displacement ........... 43
8.3 Focusing ...................................................... 44
8.4 Tubes ............................................................ 46
6
8.5 Eyepieces .................................................... 47
8.6 Objectives ................................................... 48
7
8.7 The function keys for Leica DM3000 ..... 49
8.8 Light Sources ............................................. 52
8
8.9 Aperture Diaphragm ................................. 53
8
8.10 Field Diaphragm ......................................... 54
8
9.
Contrasting Methods ................................ 55
9.1 Transmitted Light ....................................... 55
9.1.1 Brightfield ......................................... 56
9.1.2 Phase Contrast ................................ 57
9.1.3 Darkfield ........................................... 57
9.1.4 Oblique Illumination ....................... 58
9.1.5 Polarization ...................................... 58
9.1.6 Differential
Interference Contrast .................... 59
9.2 Fluorescence .............................................. 61
10. Measurements with the Microscope ... 62
10.1 Linear Measurements .............................. 62
10.2 Thickness Measurements ....................... 63
10.3 Differentiation of Gout / Pseudo Gout ... 64
11. Troubleshooting ......................................... 66
12. Care of the Microscope ........................... 70
12.1 Dust Cover .................................................. 70
12.2 Cleaning ....................................................... 70
12.3 Handling Acids and Bases ...................... 71
12.4 Changing Fuses .......................................... 71
13. Essential Wear and Spare Parts ............ 72
14. Retrofitting Components .......................... 73
14.1 Fitting the Filter Magazine
(Transmitted Light) .................................... 73
14.2 Equipping the Condenser Disc ................ 73
15. Index ............................................................ 76
16. EU Declaration of Conformity ................. 78
Contents
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