Agilent Technologies ZORBAX Guide D'utilisation page 5

Column operating Parameters: pH and Temperature
Phase
ZORBAX Eclipse Plus C18
and C8, Eclipse XDB-C8,
XDB-C18, XDB-Phenyl,
Poroshell 120 EC-C18
and EC-C8, ZORBAX
Bonus-RP, HC-C18(2) and
TC-C18(2)
Poroshell HPH
ZORBAX Extend C18
ZORBAX ODS, ZORBAX
Phenyl, ZORBAX Eclipse
Plus Phenyl-Hexyl,
Poroshell 120 PFP,
Poroshell 120 Phenyl-
Hexyl, ZORBAX XDB-CN,
ZORBAX Eclipse PAH,
All Pursuit and Polaris
bonded phases.
ZORBAX Eclipse PAH
Poroshell 120 SB-C18,
StableBond C18, ZORBAX
Rx-C8, ZORBAX SB-Aq,
ZORBAX SB-Phenyl,
SB-C3, Poroshell 300,
300SB-C3
ZORBAX TMS
Note: All silica-based packings have some solubility in pH >6 aqueous mobile
phases. When using silica-based columns at pH >6, best column lifetime is
obtained at lower temperatures (40 °C max) using low buffer concentrations in
the range of 0.01 to 0.02 M. Operating at extreme ends of pH and temperature
ranges will have a significant impact on column lifetime.
recommended
pH range
pH 2.0 to 9.0
pH 2.0 to 11.0
pH 2.0 to 11.5
pH 2.0 to 8.0
pH 2.0 to 8.0
pH 1.0 to 8.0
pH 2.0 to 7.0
ENGLISH
maximum operating
temperature
60 °C
60 °C
60 °C (40 °C at high pH)
60 °C
60 °C at pH <6.0
90 °C (StableBond C18 and
Poroshell 120 SB-C18)
80 °C (Rx-C8 and SB-Aq,
SB-C3 and SB-Phenyl)
70 °C for pH <5.0; 40 °C
for pH 5.0 to 8.0 (Poroshell
300 and 300SB-C3)
60 °C