Agilent Technologies ZORBAX Guide D'utilisation page 3

using Your Column
installation
• The direction of flow is marked on the column.
• 1.8 µm columns (ZORBAX RRHT, ZORBAX RRHD) can only be operated
in the direction flow marked on the column.
• Agilent recommends using Polyketone fittings (5042-8957) for columns
up to 600 bar, and A-Line Quick Connect assembly (5067-5961 for
0.075 x 105 mm) or Quick Turn Fitting (5067-5966) for columns that
will be operated at UHPLC pressures. Be sure to order the right
capillary connection for your fitting.
Learn more at www.agilent.com/chem/a-linefittings.
•
Polyketone fitting,
p/n 5042-8957
Column conditioning
Every column is tested before shipment and shipped in the test eluent.
So, for first use, rinsing with water is not necessary. If mobile phase
additives are used (such as buffers or ion-pair reagents) it is advisable to
do an intermediate flush with a mobile phase of the correct composition,
but without these additions. Flushing with 10 to 20 column volumes should
help in transitioning to your mobile phase. For shorter chain chemistries
(C8, Phenyl, CN, for example), care should be taken to make sure the
column has been properly equilibrated prior to use. This will ensure
reproducibility and help prevent retention time drifting.
important safety considerations
• All points of connection in liquid chromatographic systems are potential
sources of leaks. Users should be aware of the toxicity or flammability
of their mobile phases.
• Because of the small particle size, dry column packings are respirable.
Columns should only be opened in a well-ventilated area.
• Please adhere to operating pressure limits noted for each column
(see chart). Exceeding these limits will compromise chromatographic
performance and could be unsafe.
A- Line Quick Connect
assembly, p/n 5067-5961
ENGLISH
Quick Turn fitting,
p/n 5067-5966