Adjustment - Thermo Scientific Finnpipette Novus 46200000 Guide D'utilisation

A new tip is fi rst pre-wetted 3-5 times and a series of ten pipettings are done with both volumes.
A pipette is always adjusted for delivery (Ex) of the selected volume. Measuring volumes taken
from balance is not allowed.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits in the Table 1.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
TABLE1: Maximum permissible errors according ISO8655
Range Volume
1-10 µl
5-50 µl
10-100 µl
30-300 µl
100-1000 µl
0,5-5 ml
1-10 ml

Adjustment

Two point calibration
Normally the two-point calibration should be used.
1. Do the pipetting series with both max. and min. volumes.
2. Calculate the actual results.
3. Press MENU and select options with SCROLLKEY and accept with OK.
4. Select CALIBRATE and press OK.
5. Select two points and confi rm with OK. The target max. and target min. volumes are shown
on the display.
6. Press EDIT and change the actual max. volume with the SCROLLKEY and accept with OK.
7. Change the actual min. volume with the SCROLLKEY and accept with OK.
8. A text SAVE? appears on the display.
9. Accept with YES
10. The adjustment has been changed.
One point calibration
The one point calibration can be used if a single specifi c volume must be calibrated. The calibration
volume can be selected from the entire volume range. Note that the inaccuracy of other volumes
changes also and the performance for other volumes cannot be assured.
1. Do the pipetting series with calibration volume.
2. Calculate the results.
3. Press MENU and select options with SCROLLKEY and accept with OK.
4. Select CALIBRATE and press OK.
5. Select one point and confi rm with OK. The calibration volume is shown on the display.
6. Press EDIT and change the calibration volume with the SCROLLKEY and accept with OK.
7. Change the actual volume with the SCROLLKEY and accept with OK.
8. A text SAVE? appears on the display.
9. Accept with YES
10. The adjustment has been changed.
Inaccuracy
μl μl
10 ±0.120
1 ±0.120
50 ±0.50
5 ±0.50
100 ±0.80
10 ±0.80
300 ±4.0
30 ±4.0
1000 ±8.0
100 ±8.0
5000 ±40.0
500 ±40.0
10000 ±60.0
1000 ±60.0
%
±1.2
±12
±1.0
±10
±0.8
±8.0
±1.3
±13
±0.8
±8.0
±0.8
±8.0
±0.6
±6.0
11
Imprecision
s.d. μl cv%
0.080 0.8
0.080 8.0
0.20 0.4
0.20 4.0
0.30 0.3
0.30 3.0
1.5 0.5
1.5 5.0
3.0 0.3
3.0 3.0
15.0 0.3
15.0 3.0
30.0 0.3
30.0 3.0