Calibration And Adjustment - Thermo Scientific Finnpipette F3 Guide D'utilisation

3. Deliver the preset volume by gently depressing the push button to the first stop. Hold the
push button at the first stop. Some liquid will remain in the tip and this should not be included
in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into the
container.
Repetitive technique
The repetitive technique offers a rapid and simple procedure for repeated delivery of the same
volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly re
lease the push button. This action will fill the tip.
Withdraw the tipfrom the liquid touching against
the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push
button at the first stop. Some liquid will remain in
the tip and this should not be included in the delivery.
4. Continue pipetting by repeating steps 3 and 4.
Pipetting of heterogeneous samples
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress the
push button to the first stop, making sure the tip
is well below the surface.
2. Release the push button slowly to the ready position.
This will fill the tip. Keep the tip in the solution.
3. Depress the push button to the first stop and
release slowly. Keep repeating this procedure
until the interior wall of the tip is clear.
4. Finally, depress the push button all the way to the
second stop to completely empty the tip.

Calibration and adjustment

All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with
distilled or deionized water using the forward pipetting technique. It should be noted that the
use of other pipetting techniques may affect the calibration results. The pipettes are constructed
to permit re-adjustment for other pipetting techniques or liquids of different temperature and
viscosity.
Device requirements and test conditions
An analytical balance must be used. The scale graduation value of the balance should be chosen
according to the selected test volume of the pipette:
Volume range
under 10 µl
10-100 µl
above 100 µl
Test liquid: Water, distilled or deionized, "grade 3" water conforming ISO 3696. Tests are done
in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air between 15°C
to 30°C. The relative humidity must be above 50%. Especially with volumes under 50 µl the
air humidity should be as high as possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are recommended.
readable graduation
0.001 mg
0.01 mg
0.1 mg
7
1 2 3 4
1 2 3 4 5