Adjustment - Thermo Scientific Finnpipette Guide D'utilisation Et D'entretien

DEVICE REQUIREMENTS AND TEST CONDITIONS
An analytical balance must be used. The scale graduation value of the balance should be
chosen according to the selected test volume of the pipette:
Volume range
under 10 µl
10-100 µl
above 100 µl
Test liquid: Water, distilled or deionized, "grade 3" water conforming ISO 3696. Tests are
done in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air
between 15°C to 30°C.
The relative humidity must be above 50%. Especially with volumes under 50 µl the air
humidity should be as high as possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are recommended.
PROCEDURE TO CHECK CALIBRATION
The pipette is checked with the maximum volume (nominal volume) and with the
minimum volume. A new tip is first pre-wetted 3-5 times and a series of ten pipettings is
done with both volumes. A pipette is always adjusted for delivery (Ex) of the selected
volume.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits in the Table 1.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
TABLE 1: Maximum permissible errors according ISO8655
Range Channel
1-10 µl 8, 12
5-50 µl 8, 12, 16
30-300 µl 8, 12

ADJUSTMENT:

Adjustment is done with the service tool.
1. Place the service tool into the openings of the calibration nut at the top of the handle.
2. Turn the service tool clockwise to increase, or counterclockwise to decrease the
volume.
3. After adjustment check the calibration according to the instructions above.
readable graduation
0.00 1 mg
0.01 mg
0.1 mg
Volume
µl
10
1
50
5
300
30
Inaccuracy
µl %
±0.24 ±2.4
±0.24 ±24
±1.0 ±2.0
±1.0 ±20
±8.0 ±2.7
±8.0 ±26.7
7
Imprecision
s.d.µl cv%
0.16 1.6
0.16 16
0.4 0.8
0.4 8.0
3.0 1.0
3.0 10.0
8