Thermo Scientific Finnpipette Novus 46200000 Guide D'utilisation page 9

1. Dip the tip under the surface of the liquid in the reservoir and press the trigger.
This action will fi ll the tip and the fi rst volume appears on the display.
2. Withdraw the tip from the liquid touching it against the edge of the reservoir to remove
excess liquid.
3. Deliver the fi rst volume by pressing the trigger. The display shows the next volume.
4. Continue dispensing by repeating step 3. After last step a text BLOWOUT is displayed.
5. To empty the tip completely, press trigger.
6. If necessary, change the tip and continue pipetting.
Note: The pipette can be emptied at any time by pressing CANCEL (left selection key)
Mix + Dilute
Select the fi rst pipetting volume by pressing the scroll key up or down. Accept the volume with
OK. Next select the second volume (VOL 2) with scroll key and accept with OK. Then select the
pipetting speeds.
1. Dip the tip under the surface of the fi rst liquid in the reservoir and press the trigger. The
fi rst volume is drawn in to the tip. A text AIR appears on display.
2. Withdraw the tip from the liquid touching it against the edge of the reservoir to remove
excess liquid. Press trigger again to aspirate air buffer.
3. Dip the tip under the surface of the second liquid and press the trigger.
The second volume is drawn in to the tip.
4. Withdraw the tip from the liquid.
5. To deliver the both volumes, press the trigger. A text MIX appears on the display.
6. By pressing trigger the pipette starts to pipette ca. 70 % of the total volume several times as
long as the trigger is held pressed.
7. After releasing the trigger the pipette stops after next dispensing and a text BLOWOUT
appears to display.
8. Withdraw the tip from the liquid and press the trigger and hold it down to empty the tip.
9. Release the trigger to return to the ready position.
10. If necessary, change the tip and continue pipetting.
Manual
With manual mode it is possible to measure volumes. In manual mode only slower speeds are
available to ensure a quick stop. First select a limit for total volume, the default is the max. volume.
Then select the pipetting speeds and zero volume appears to the display.
1. Dip the tip under the surface of the liquid in the reservoir and press the trigger.
The liquid is drawn in to the tip and the actual volume is shown on the display.
2. The pipetting direction can be changed with IN/OUT key (Left selection key).
3. To deliver the liquid selected the down direction and press the trigger.
4. To deliver out the rest of the liquid, press the trigger and hold it down.
5. If necessary, change the tip and continue pipetting.
Note: The volume display can be reset to zero at any time by pressing RESET with right selection
key.
Seq + Aspirate
The sequential aspirate mode enables serial aspirating of different volumes. First choose the
number of volumes (max. 20) with SCROLLKEY and accept with OK. Vol 1 appears to display and
highest possible volume is fl ashing. Select the fi rst volume with SCROLLKEY and accept with
OK. Now Vol 2 appears to display and highest possible volume left is fl ashing. Select the second
volume with SCROLLKEY and accept with OK. After selecting the last volume the total volume is
shown on display and speed in is fl ashing for selecting the pipetting speeds. After selecting the
speeds the fi rst volume is shown on the display and the pipette is ready for pipetting.
1. Dip the tip under the surface of the liquid and press the trigger.
This action will take up fi rst volume and the next volume appears on the display.
2. Withdraw the tip from the liquid touching it against the edge of the reservoir to remove
excess liquid.
3. Dip the tip under the surface of the next liquid and press the trigger. This action will take
up current volume and the next volume appears on the display.
4. Repeat steps 2. and 3. until last volume is drawn in to the tip.
The total volume is shown on the display.
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