IV. GENERAL CARE AND CLEANING
WARNING! Acrylic is not resistant to aromatic or halogenated hydrocarbons, ketones or esters.
Organic solvents cause acrylic to "craze" or crack. Do not use ethanol or other organic solvents
to clean the unit.
• Do not autoclave, bake or microwave the unit.
• Before using, clean and dry the unit. Clean with DISTILLED WATER ONLY; dry parts with clean,
lint-free lab wipes or, preferably, air dry. Use care when cleaning or drying the unit near the
platinum wire. The connectors should be clean and dry before usage or storage.
• Do not use abrasive creams or scourers.
• Do not use cleaning brushes in the electrode area.
• A thorough rinse with distilled water is all that is generally required to clean the unit after use.
A mild detergent may also be used. Acrylic can also be exposed to a mild bleach solution (10:1).
In addition RNAse removal products are also safe for acrylic.
Gel tray
All STARLAB gel trays are made from UV transmissible acrylic, which allows a stained agarose gel to be
viewed directly in the tray on a transilluminator. An incorporated fluorescent ruler aids in the calculation
of relative mobility of DNA fragments.
NOTE: care should be taken to avoid pouring freshly melted agarose directly into the gel tray, as this
may damage the tray. Always allow agarose to cool to 60
Combs
Combs are available in thicknesses of 1.0mm and 1.5mm. Comb spines are acrylic and the teeth are
polycarbonate. Combs designated "Microtitre" are compatible for use with a multi-channel pipettor.
Each comb is labelled with its thickness, number of teeth and catalogue number. Please see Chapter 10,
Comb Specifications, for details of the combs available for each device.
Buffer Tank
The buffer tank holds the buffer and gel tray. Current is passed from the power supply through the leads
to the cover connectors, through the platinum wires and into the buffer and from there into the gel.
The cover incorporates safety rings that prevent human contact with the electrode connections to
prevent electrical shock if the power supply is not turned off prior to removing the lid. Users should not
attempt to operate the system without the lid in place. The electrode banana plugs are colour-coded
red and black to ensure that the gel is placed in the correct orientation in the tank [almost all
electrophoretic separations of biomolecules run from the black electrode (negative) to the red
electrode (positive)]. The connection between the banana plug and the electrode wire is sealed with
a proprietary composite that eliminates corrosion of the connection. The electrode wires are 0.254mm
platinum and are secured to the inner walls of the tank. Care should be taken not to damage the
electrodes when cleaning the unit.
StarPhoresis Mini and Wide Mini Gel Horizontal Electrophoresis Systems
C before pouring into the tray.
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