Specifications; Casting The Gel - StarLab Phoresis N2007-0810 Manuel D'utilisation

V. SPECIFICATIONS
TABLE B: General specifications
Gel dimensions (w x l)
System
dimensions
(l x w x h)
Tray configuration
Comb thickness
Comb
configurations
Voltage limit
Max. buffer volume
VI. CASTING THE GEL
1. Place the gel tray on a flat, level surface. It is important to pour the agarose gel on a level surface
to prevent uneven migration of samples through the gel. If casting tape is to be used, tightly secure
the tape over the open ends of the tray. If using a gasketed STARLAB tray place the tray into the
chamber of the device so that the gasketed ends press against the walls of the buffer chamber.
Make sure that the gel tray is pressed all the way down and rests on the unit's gel tray platform.
1.1 If the optional Adjustable Casting Platform is to be used, ensure that the platform is level using
the three point levelling system and place the tray into the casting platform with the open ends
facing the gasket. Position the centre hole of the sliding arm of the caster over the correct hole in
the base and insert the cam into the hole in the arm and through to the hole in the platform with
the cam pin facing the tray. Rotating the cam pin 180
gaskets into position on either side of the tray. If the tray is correctly positioned with the gaskets,
it should not move when gently pushed from the side.
2. Prepare agarose as described in your individual application.
3. IMPORTANT: to avoid damage to UVT tray, always cool agarose to 60°C before pouring into tray.
Any bubbles that form while pouring may be popped with a small glass pipette or the edge of the
comb. Place combs into the comb slots on the gel tray being careful not to create bubbles along the
teeth of the comb. If the entire gel length is required for separation, place one comb in the first
comb slot on the tray. To separate more samples on one gel, use both comb slots on the tray. Allow
gel to solidify for 30 minutes.
6
StarPhoresis Mini and Wide Mini Gel Horizontal Electrophoresis Systems
N2007-0810
7 x 8 cm
17.6 x 12 x 9.5cm
2 comb slots
5, 6, 8, 10 or 12 wells
& Preparative Comb
150 volts
400ml
N2009-1110
9 x 11 cm
22 x 15 x 9.5cm
2 comb slots
1.5mm, plus optional 1.0mm
5, 8, 10, 12, 14 wells
& Preparative Comb
150 volts
600ml
will force the tray forward and bring both
o
N2012-1410
12 x 14cm
24.5 x 18 x 9.5cm
4 comb slots
8, 16, 20, 24, MT9,
10, 20, 30, 40 & M25
MT12, MT25 wells
& Preparative Comb
150 volts
800ml
N2014-1010
14.4 x 10.2cm
17 x 17 x 10cm
2 comb slots
wells
150 volts
600ml