Operation - StarLab Phoresis N2007-0810 Manuel D'utilisation

VII. OPERATION
1. Once the agarose has solidified, carefully remove the casting tape from the gel tray or the gel tray
from the casting platform. If a gasketed tray is used, carefully lift the tray out of the chamber, turn
it 90 and replace it into the chamber. Remove casting comb(s) slowly to prevent any tearing of the
o
wells.
NOTE: If a high percentage agarose gel is cast it may be difficult to remove the casting comb from
the tray. If resistance is felt when removing the casting comb, place the gel tray into the buffer tank
and add buffer before removing the casting comb.
Place the tray onto the platform of the tank. Be sure the gel tray is properly oriented in the tank; the
first comb slot should be closest to the black (cathode) electrode. The banana plugs on the tank are
colour coded to help ensure proper orientation.
2. Pour the buffer into the tank. The level of buffer should cover the gel by at least 2-3mm (see Table B:
General specifications for buffer volume guidelines). A fill line is located on the outside wall of the
tank for easy reference. Too little buffer may cause the gel to dry out during the run, while excess
buffer may slow DNA migration in the gel.
3. Load your samples on the gel. To load samples, angle the pipette tip into the well and slowly
underlay sample into the well. Take care not to push pipette tip through the bottom of the sample
well as this will result in significant well-to-well leakage of samples
4. Slide the cover onto the electrophoresis buffer tank, insuring complete connection of the tank's
banana plugs to the female connector ends of the power cords that are fixed to the cover. The cover
acts as a safety inter-lock to prevent accidental shock during operation.
5. Attach the power cords to the power supply. Turn on the power supply and set to the appropriate
voltage level and begin electrophoresis.
Recommended running conditions are 5 volts/cm of inter-electrode distance, see TABLE C below.
TABLE C: Recommended run conditions
Model
N2007-0810
N2009-1110
N2012-1410
N2014-1010
6. Run the gel for the appropriate amount of time for the specific sample being analysed or until the
dye front has migrated to approximately 5mm from the end of the gel. Turn off power supply and
detach the power cords. Slide the cover back to remove. Lift the gel tray out of the buffer tank and
proceed with visualisation.
7. It is important to rinse the tank with distilled water after every use in order to keep it clean. It is
recommended to allow the tank to air dry, rather than drying with a wipe or towel, to avoid
damage to the electrode wires.
StarPhoresis Mini and Wide Mini Gel Horizontal Electrophoresis Systems
Distance between
electrodes
18cm
19cm
22.5cm
17cm
Voltage
90V or 110V constant
95V or 110V constant
112.5V or 110V constant
85V
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