Evaluation Using The Rqflex Plus Reflectometer; Encoding The Measuring Device; Setting Up A Control Test; Limit Of Determination - Miele professional TK/1 Mode D'emploi

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Evaluation using the RQflex plus reflectometer

Encoding the measuring device

Setting up a control test

Limit of determination

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The Merck RQflex plus reflectometer with cuvette adapter
can evaluate the approximate quantitative protein content
within the colour range from 10 to 60 µg protein/ml.
Encoding of RQflex plus is by means of the two barcode
strips supplied with the test kit and is described in the
operating instructions for the reflectometer.
When measuring with the RQflex plus a control test must
always be carried out in addition to the test solutions being
measured.
To run a control test, add 1 ml of ready-to-use SDS solution
to a reaction bottle with reagent A and mix by making
circular movements.
The control test can be used for taking a series of
measurements within a period of up to 30 minutes.
Protein content of between 10 µg and 60 µg protein/ml can
be detected using this method.
This means that the limit of determination when using 2 ml
SDS solution for the assay is approx. 20 µg protein or when
using 3 ml SDS solution approx. 30 µg protein for the total
test volume, etc.