Leica Kreatech FISH probes Mode D'emploi page 19

Kreatech FISH probes
12. 70% acetic acid (in deionized H
13. DAPI counterstain (LK-095A (0.1 μg/ml) or LK-096A (1 μg/ml))
14. Counterstain diluent (LK-097A)
15. Formamide
16. Ethanol 100%, 85% and 70%
17. Fixogum (LK-071A) or rubber cement
Material required, but not supplied: equipment:
1. ThermoBrite (TS-01/02)
2. Incubator at 37 °C
3. Water bath with accurate temperature from 37 °C to 90 °C
4. Plastic or glass Coplin jar
5. Variable micropipettes (1 μl - 200 μl)
6. Fluorescence microscope equipped with suitable filters (see
recommendations for fluorescence microscopy)
Warnings and precautions:
1. For professional use only. In case of emergencies check SDS
sheets for safety information.
2. DNA probes and hybridization buffers contain formamide which is
a teratogen; do not inhale or allow skin contact. Wear gloves and
a lab coat when handling DNA probes and DAPI counterstain.
3. All materials should be disposed of according to your institution's
guidelines for hospital waste disposal.
4. Labelling according Regulation OSHA HazCom Standard (2012)
requirements.
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