Operation - StarLab StarPhoresis N2510-1010 Manuel D'utilisation

5. To clamp the assembly to the upper buffer chamber
slide the clamp bars towards the middle. Tighten the
wing nuts until a seal is formed between the gasket
and the glass. The hollow gasket allows for a superb
seal without over-tightening the wing nuts. Over-
tightening may cause the glass to break.
6. If casting and running two gels, repeat steps 4 to 6 for
the second gel plate assembly. If you wish to run only
one gel, secure the blocking plate to the second side.
Note: a combination of two gel assemblies or one gel
assembly and the blocking plate are necessary to form
the walls of the upper buffer chamber.
7. Lift the assembled upper buffer chamber and turn the
casting base over. Turn the cams so that the handle
is pointing up and pull out. Place the upper buffer
chamber on the gaskets. Note: A protective plastic film
is left on the gaskets for shipping. A piece of clear tape
has been fastened to the end of the film to assist the
user in removing it
8. Insert the cam pins and simultaneously turn the handles
one half turn to tighten the assembly down onto the
gasket base. Once the Upper Buffer Chamber assembly
has been secured onto the casting base an initial leak test using a small amount of water is
recommended – add 2ml–3ml of water and let stand for 2 minutes. If no leakage is visible, empty water
and proceed with gel casting.
VII. OPERATION
Casting the Gel
1. To ensure reproducibility and uniform polyacrylamide cross-linking we recommend de-ionizing,
de-gassing and filtration of acrylamide gel solutions prior to use. Acrylamide solutions should be
stored in a cool, dark environment such as a refrigerator and allowed to reach room temperature prior
to pouring. Avoid exposure to heat and sunlight. Polymerization conditions should be adjusted to
effect polymerization within about 5–10 minutes. Test a small volume in a vial prior to pouring the gel.
As a rough guide, 100ml of degassed 6% acrylamide gel will set in about 5 minutes at room
temperature when gently mixed with 450μl of freshly prepared 10% (w/v) Ammonium persulphate
plus 200μl TEMED. The setting time increases to about 10 minutes if the TEMED volume is reduced
to 100μl and to approximately 15 minutes with 75μl. The amount of catalysts may need to be reduced
under warm conditions. Do not pour under direct sunlight.
Prepare the appropriate volume of acrylamide gel solution using Table B below as a guide. These
volumes have been calculated using the glass and spacers provided by STARLAB, and subtracting the
volume of the spacers and the notch. The volumes are approximate.
StarPhoresis 2-Gel Mini and Wide Mini Vertical Electrophoresis Systems
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