StarLab StarPhoresis N2510-1010 Manuel D'utilisation page 9

• Load the samples using a gel loading pipette tip (see STARLAB's range of gel-loading tips).
See Appendix IX. Comb/Spacer Specification for approximate well volumes. If possible, avoid taking
liquid from the pellet area at the bottom of the tube. During sample loading the pipette tip should be
1 2mm above the bottom of the well to minimise dilution of the sample and to keep the sample as a
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tight layer.
• Fill unused wells with the equivalent volume of sample buffer to maintain uniform electrical
resistance across the gel.
• Add buffer to the lower buffer chamber to approximately 2
Fill Line as a guide. The bottom end of the gel assembly should be in contact with the running buffer
(see also VII, Gel Casting, point 4).
• Set the safety lid onto the unit so that the power cords are connected in the proper position (red to
red, black to black)
Integrated water cooling system
All StarPhoresis 2-Gel Electrophoresis Systems have an integrated water cooling system. Sometimes
cooling is needed when running gels at a higher current or when the bioactivity of an enzyme has to be
preserved. Heating of the gel can cause smiling and other problems with the resolution of protein bands.
This is particularly pronounced on larger gels. We recommend running coolant or water through the cool-
ing core in the upper buffer chamber. When ramping up voltage or current, consider at least tap water
cooling.
To utilise Cooling System
• Attach a separate piece of 3/8" ID clear flexible lab tubing to each hose barb on the upper buffer
chamber marked as 'in' and 'out'
• Attach the tubing from the cathode (black) side of the unit, marked 'in' to either a cold-water tap or a
re-circulator/chiller.
• Water flow should not exceed 2 litres per minute at 30 psi.
• Attach the drain tube on the anode (red) side marked as 'ou' to the re-circulator chiller or put into the
sink drain.
• Turn on the water. Once the water has started to circulate through the system, connect the power
cords to the power supply
Starting and ending the run
Starting
• Connect the chamber to the power supply and connect the power supply to the main electrical
source. Turn all settings to zero before turning on the main source of electricity. Adjust the controls to
the desired settings. Follow manufacturer's instructions.
Ending
• Turn power supply settings to zero, turn off the main electrical source and disconnect the power
cords. Turn off the water (if using cooling system).
• Remove the lid by pushing on the acrylic alignment pins protruding through the top of the lid with
your thumbs. Slide and lift the upper buffer chamber out of the lower buffer chamber and drain
buffer chambers separately.
StarPhoresis 2-Gel Mini and Wide Mini Vertical Electrophoresis Systems
3mm above the base of the gel using the
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