Fluorescence - Leica DM5500 B Manuel D'utilisation

9. Contrasting Methods for Leica DM5500 B/DM6000 B

9.2 Fluorescence

• Switch to the fluorescent light axis (FLUO) by
pushing the TL/IL button.
• Insert a specimen and select an appropriate
objective.
• The current fluorescence cube is indicated on
the display of the LeicaScreen, menu page
'Status'.
• Closing the incident light shutter protects your
specimen from fading.
Do so by pressing the TL-SHUTTER variable
key.
(For key occupation please see "Identification
Sheet".)
The display of the Leica SmartTouch indicates
the symbol:
Fig. 78 Inserting the Booster Lens
76
Selecting the fluorescence filter cube:
Leica SmartTouch – navigation item
Variable function keys at the stand and at
the SmartMove: Cube CW or Cube CCW
Leica STP6000
Software Leica Application Suite, module:
Acquire
• Focus on the image with the turning knob on
the SmartMove, STP6000 or with the focus
wheel and adjust the illumination with the INT-
keys.
• The fluorescence intensity can be increased
using the Booster Lens on the right side of the
stand (Fig. 78). It is recommended to insert the
Booster Lens into the front receptacle.
• For multifluorescence, a manual Excitation
Manager can be used. In this case, the
Excitation Manager is inserted into the right
side of the stand up to the last stop (Fig. 79). It
is recommended to insert the Excitation Man-
ager into the front receptacle.
Fig. 79 Inserting the Excitation Manager