Interference Contrast; Fluorescence - Leica DM5500 B Manuel D'utilisation

10. Contrasting Methods for Leica DM6000 M
10.1.4 Interference Contrast (IL)
• Switch to the incident light axis (IL) by push-
ing the TL/IL button.
• Insert a specimen and select an appropriate
objective.
• Select the DIC contrast method.
Do so by pressing the DIC variable key.
Alternatively: Press the CHANGE RL
variable key.
(For key occupation please see "Identification
Sheet".)
The display of the Leica SmartTouch, menu
page 'Status' indicates DIC.
• The ICR filter cube (containing polarizer and
analyzer) is automatically brought into the beam
path on the incident light axis.
Alternatively:
• Rotate the ICR polarizer (80.1) and the IC/P
analyzer (81.1) on the stand manually into the
light path.
• Objectives and condenser prisms are swung
into the beam path automatically.
• For fine adjustment, rotate the knurled wheel
(80.2).
80

10.1.5 Fluorescence

• Switch to the incident light axis (IL) with the
function key TL/IL.
• Insert a specimen and select an appropriate
objective.
• If the last used IL-mode was fluorescence, the
current fluo filter cube is displayed on the
Leica SmartTouch.
Selecting the fluorescence filter cube:
Leica SmartTouch – navigation item
Variable function keys at the stand and at
the SmartMove: Cube CW or Cube CCW
Leica STP6000
Software Leica Application Suite, module:
Acquire
• Focus on the image with the turning knob on
the SmartMove, STP6000 or with the focus
wheel.