Fluorescence - Leica DMLS Mode D'emploi

Adjustment crossed position λ and λ/4 com-
pensator
Cross polarizers: Remove the object or find an
empty area of the specimen. Rotate the polarizer
until you reach the maximum extinction position
in the eyepiece. Insert the whole- or quarter-
wave compensators above the polarizer (28.2)
and rotate to the left, roughly as far as the stop.
The disc (9.6) can also be equipped with a
whole- and quarter-wave compensator.
Possible errors
Polarizers damaged (discoloured) by powerful
light sources or dirty. Objectives or condenser
strained through mechanical damage or wrong
condenser.
Beamsplitter or filter between the polarizers.
Mounting medium or specimen slide or cov-
erglass birefringent. Further sources of error
→ p. 33.
Fig. 29 Lamphousing 106 (with 12 V 100 W halogen lamp)
1 Screw for opening Lamphousing 106, 105/2 and 107, 2, 3 X/Y
lamp centration (holes for Allen centering keys or 3 mm
screwdriver), 4 Collector focusing, 5, 7 Clamp screw, 6 Filter
holder (spacer)* (Pos. 2 – 4 do not apply for Lamphousing
105/2 and 107)
1
3 2
38
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4 5 6 7

Fluorescence

!
Achtung:
Switch on the lamp by the external power unit.
Hg lamps take a few minutes to achieve their full
intensity, and do not ignite when hot!
Move filter cube into the light path with the slide
(31. 1 0; 14.3). Open the light trap (31.9).
Attention:
Make sure to adjust the light sources
immediately, then form an image by one of
the following methods (30; 32), does not apply
for Lamphousing 105/2 and 107 (without
centering facility): Danger of glare! Never
look into the direct light path or switch on
incident light brightfield reflectors when
using Hg or Xe lamps!
Fire hazard! Keep
surfaces!) at least 10 cm (4") away from
inflammable objects such as curtains,
wallpaper or books!
Fig. 30 Lamphousing 106
Reflection of the lamp filament, greatly schematized: in reality
the reflection is extremely low in contrast, the bright overlap
area is wider and less defined.
lamphousings (hot