Leica DM5500 B Manuel D'utilisation page 84

11. Trouble Shooting
Problem
Polarization
No polarization contrast is possible.
Fluorescence
The image is completely dark (no fluorescence).
The fluorescence is too weak.
84
Cause/Remedy
Bring the polarizer and analyzer into cross po-
sition until they reach maximum darkness
(without specimen) (→ p. 74, 79).
Open the shutter (→ p. 76).
Select the incident light axis (IL) (→ p. 48).
Check the antigen-antibody combination.
Insert a new lamp (→ p. 26f).
Insert the Booster Lens (→ p. 36).
Center the lamp (→ p. 54ff).
Insert a new lamp (→ p. 26f).