Transmitted Light; Bright Field; Polarization - Leica DM5500 B Manuel D'utilisation

10.2 Transmitted Light

10.2.1 Bright Field (TL)
• Switch to the transmitted light axis by pushing
the TL/IL button.
• Select the BF (bright field) contrast method.
Do so by pressing the BF TL variable key.
(For key occupation please see "Identification
Sheet".)
The display of the Leica SmartTouch, menu
page 'Status' indicates BF TL.
• Insert a transmitted light specimen.
• Select an appropriate objective.
• Focus on the image with the turning knob on
the SmartMove, STP6000 or with the focus
wheel and adjust the illumination with the INT-
keys.
10.2.2 Polarization (TL)
• Switch to the transmitted light axis (TL) by
pushing the TL/IL button.
• Select the POL (polarization) contrast method.
Do so by pressing the POL variable key.
Alternatively: Press the CHANGE TL
variable key.
(For key occupation please see "Identification
Sheet".)
The display of the Leica SmartTouch, menu
page 'Status' indicates POL.
10. Contrasting Methods for Leica DM6000 M
Manual procedure:
• Turn the polarizer on the underside of the con-
denser in the light path (Fig. 75). Make sure
that the red index point on the front of the
polarizer is aligned with 0.
• Insert the analyzer into the left side of the
stand (76.1).
• Bring the polarizer and analyzer into cross po-
sition until they reach maximum darkness.
• Insert a specimen and select a low-magnifi-
cation objective.
Motorized procedure:
• After selecting the POL contrast method, the
condenser automatically switches to the posi-
tion of the polarizer. The analyzer cube is also
automatically brought into the light path.
Combined procedure:
• For the Leica DM6000 M microscope, it is pos-
sible to combine mechanical and motorized
components.
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