Optika Italy B-510 Serie Manuel D'instructions page 53

15.3 Use of the microscope (B-510LD series)
The filter turret is provided with 4 positions.
• In each of the four positions a fluorescence filter can be in-
serted, which can be selected from the options shown in the
table below.
• You can always add an additional filter after the first in-
stallation (see section 8.6).
• If all four turret positions are full, the observation in
transmitted light will be affected by the presence of the
fluorescence filter.
1. Turn on the main switch ①. (Fig. 100)
• For the model B-510LD4D: turn on the microscope using
the main switch on the right side of the frame. (Fig. 53)
2. Move the filter selector ② to one of the available positions
until the click stop. (Fig. 101)
• When the filter is in the correct position, the dedicated LED
lights up.
3. Adjust to the desired brightness by rotating the dial ①.
• When switching the fluorescence filter, the LED light
goes out. This is not a defect.
FILTER EXCITATION
NAME FILTER
M-1223 325-375 nm
M-1223.1 340-390 nm
M-1222 390-420 nm
M-1220 455-495 nm
M-1220.1 455-495 nm
M-1221 510-550 nm
M-1221.1 510-550 nm
M-1228 582-603 nm
M-1224 (*) 590-650 nm
M-1225 (*) 595-645 nm
M-1226 (*) 623-678 nm
M-1227 (*) 720-760 nm
(*) If the use of a camera is needed, please order it by specifying with "AR GLASS" in order to observe above
650nm.
DICHROIC EMISSION
MIRROR FILTER
415 nm 435LP nm
405 nm 420-470 nm
440 nm 450LP nm
500 nm 510LP nm
500 nm 518-542 nm
570 nm 575LP nm
570 nm 585-625 nm
610 nm 615-645 nm
660 nm 665LP nm
655 nm 665-715 nm
685 nm 690-750 nm
770 nm 780LP nm
Page 53
②
APPLICATIONS
• DAPI, Hoechst, NucBlue, Alexa Fluor 350, BFP, Cou-
marin
• Pacific Blue, Spectrum Blue
• GFP, Alexa Fluor 488, Calcein, SYBR Green, FITC,
Fluo-4, MitoTracker Green
• Spectrum Gold, Propidium Iodide, Ethidium Homodi-
mer I
• Texas Red, Alexa Fluor 594, mRaspberry, Zombie Red
• Cy5, Alexa Fluor 647, DRAQ5
• Alexa Fluor 660, DRAQ5
• Indotricarbocyanine, DiR
①
F ig. 100 F ig. 100
F ig. 101 F ig. 101