Optika Italy B-510 Serie Manuel D'instructions page 52

15.2 Use of the microscope (B-510FL)
1. Turn on the power supply ① for the mercury bulb and wait 5
minutes for the arc to stabilize. (Fig. 97)
2. Move the filter selector ② to one of the four available positions
until the click stop. (Fig. 98)
• The microscope has a 4-position filter holder slider. The
positions 1 and 2 are empty to house additional filters,
position 3 houses a B filter and position 4 a G filter.
F I L T E R EXCITATION
NAME FILTER
B* 460-495 nm
G* 510-550 nm
UV** 325-375 nm
V** 390-420 nm
(*) Already installed in the microscope
(**) Optional
15.2.1 Use of the shutter (B-510FL only)
The microscope is equipped with a shutter ③ located on
•
the right side of the fluorescent illuminator. (Fig. 99)
1. Close the shutter in order to interrupt the observation for a
limited time and not subjecting the sample to unnecessary
lighting in the period in which it is not observed. (Switching
off and switching on frequently the HBO lamp considerably
reduces its duration).
D I C H R O I C BARRIER
MIRROR FILTER
505 nm 510LP nm
570 nm 575LP nm
415 nm 435LP nm
440 nm 455LP nm
• GFP, Alexa Fluor 488, Calcein, SYBR Green, FITC,
Fluo-4, MitoTracker Green
• Spectrum Gold, Propidium Iodide, Ethidium Homod-
imer I
• DAPI, Hoechst, NucBlue, Alexa Fluor 350, BFP
• NucViolet, Pacific Orange, Spectrum Blue
Page 52
②
APPLICATIONS
③
①
F ig. 97 F ig. 97
F ig. 98 F ig. 98
F ig. 99 F ig. 99