Pipette Operation; Pipetting Techniques - Thermo Scientific Finnpipette F3 Guide D'utilisation

Setting the delivery volume
1. Set the delivery volume using the push button on the top of
the pipette. To increase the delivery volume, turn the push button
counterclockwise. To decrease the delivery volume, turn it clockwise.
2. Make sure that the desired delivery volume clicks into place.
3. Do not set volumes outside the pipette´s specified volume range.
Using excessive force to turn the push button outside the range
may jam the mechanism and eventually damage the pipette.
Safety Label
You can mark the pipette application, your initials, the
calibration date, etc. on the safety label. Remove the old
label with a sharp needle. Mark the new label with a pencil
and slide the label back in place.
Tip ejection
To help eliminate the risk of contamination, each pipette
is fitted with a tip ejector system. To release the tip, point
the pipette at suitable waste receptacle and press the tip
ejector with your thumb.
Push and release the push button slowly at all times particularly when working with
high viscosity liquids. Never allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone. Check for foreign
particles in the tip.
Before you begin your actual pipetting work, fill and empty the tip 2-3 times with
the solution that you will be pipetting. Hold the pipette in an upright position while
aspirating liquid. The grippy should rest on your index finger. Make sure that the tips,
pipette and solution are at the same temperature.
Forward technique
Fill a clean reagent reservoir with the liquid to be dispensed.
1 Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm and
slowly release the push button. Withdraw the
tip from the liquid touching it against the edge of
the reservoir to remove excess liquid.
3. Deliver the liquid by gently depressing the push
button to the first stop. After a delay of about
one second, continue to depress the push button
all the way to the second stop. This action will
empty the tip.
4. Release the push button to the ready position. If
necessary, change the tip and continue pipetting.
Reverse technique
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency
to foam easily. The technique is also recommended for dispensing very small volumes. Fill a
clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the
second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly
release the push button. This action will fill the
tip. Withdraw the tip from the liquid touching
it against the edge of the reservoir to remove
excess liquid.

Pipette operation

Pipetting techniques

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