Thermo Scientific Finnpipette F3 Guide D'utilisation page 8

Procedure to check calibration
The pipette is checked with the maximum volume (nominal volume) and with the minimum
volume. A new tip is first pre-wetted 3-5 times and a series of ten pipettings is done with
both volumes. A pipette is always adjusted for delivery (Ex) of the selected volume. Use of
forward pipetting technique is recommended. The maximum permissible errors are designed for
forward method. It is recommended to calibrate all channels of the multichannel pipette, but a
suitable method is also to calibrate only the edge channels. Adjustment check prior calibration is
done using one of the middle channels. It is recommended to use the ISO8655 calibration limits
presented in the following tables or user-defined specifications.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
ISO8655 calibration specifications
Thermo Scientific Finnpipette F3 specifications according to ISO8655 standard.
Finnpipette F3 Variable Volume Single Channel
Range
0.2-2 µl
1-10 µl
2-20 µl
5-50 µl
10-100 µl
20-200 µl
100-1000 µl
0.5-5 ml
1-10 ml
Finnpipette F3 Fixed Volume Single Channel
Fixed Volume
µl
1
5
10
20
25
50
100
200
250
500
1000
Volume Inaccuracy
µl µl
2 ±0.080
0.2 ±0.080
10 ±0.120
1 ±0.120
20 ±0.20
2 ±0.20
50 ±0.50
5 ±0.50
100 ±0.80
10 ±0.80
200 ±1.60
20 ±1.60
1000 ±8.0
100 ±8.0
5000 ±40.0
500 ±40.0
10000 ±60.0
1000 ±60.0
Inaccuracy
µl
±0.050
±0.125
±0.120
±0.20
±0.50
±0.50
±0.80
±1.6
±4.0
±4.0
±8.0
8
%
±4
±40
±1.2
±12
±1.0
±10.0
±1.0
±10
±0.8
±8.0
±0.8
±8.0
±0.8
±8.0
±0.8
±8.0
±0.6
±6.0
Imprecision
% s.d.µl
±5.00 0.050
±2.50 0.075
±1.20 0.080
±1.00 0.10
±2.00 0.20
±1.00 0.20
±0.80 0.30
±0.80 0.60
±1.60 1.50
±0.80 1.50
±0.80 3.00
Imprecision
s.d. µl cv%
0.040 2.0
0.040 20.0
0.080 0.8
0.080 8.0
0.10 0.5
0.10 5.0
0.20 0.4
0.20 4.0
0.30 0.3
0.30 3.0
0.60 0.3
0.60 3.0
3.0 0.3
3.0 3.0
15.0 0.3
15.0 3.0
30.0 0.3
30.0 3.0
cv%
5.00
1.50
0.80
0.50
0.80
0.40
0.30
0.30
0.60
0.30
0.30